Difference between revisions of "Part:BBa K2978201"
| Line 3: | Line 3: | ||
<partinfo>BBa_K2978201 short</partinfo> | <partinfo>BBa_K2978201 short</partinfo> | ||
| − | This part is the negative control for the efficiency terminators assay, where we tested three different sequences for < | + | This part is the negative control for the efficiency terminators assay, where we tested three different sequences for <Lactobacillus spp</i> [[Part:BBa_K2978201| K2978201]] [[Part:BBa_K2978202| K2978202]] [[Part:BBa_K2978203| K2978203]] [[Part:BBa_K2978204| K2978204]] and [[Part:BBa_K2978205| K2978205]]. |
| − | + | <br> | |
The composite part is based in constitutively express a GFP before of each terminator tested (in this specific part there is not terminator), and a RFP in a polycistronic way after the terminator. By comparing the fluorescence of each protein, the efficiency of the terminator could be determined. | The composite part is based in constitutively express a GFP before of each terminator tested (in this specific part there is not terminator), and a RFP in a polycistronic way after the terminator. By comparing the fluorescence of each protein, the efficiency of the terminator could be determined. | ||
Revision as of 11:29, 20 October 2019
Control for Terminators Measuarement in Lactobacillus spp
This part is the negative control for the efficiency terminators assay, where we tested three different sequences for <Lactobacillus spp</i> K2978201 K2978202 K2978203 K2978204 and K2978205.
The composite part is based in constitutively express a GFP before of each terminator tested (in this specific part there is not terminator), and a RFP in a polycistronic way after the terminator. By comparing the fluorescence of each protein, the efficiency of the terminator could be determined.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]