Difference between revisions of "Part:BBa K3286205:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
A high level of Vc2 riboswitch in the cell might cause some Vc2 Riboswitches not being able to be bind c-di-GMP molecules since these might get scarce. Therefore we used the mid-strength Anderson promoter to test this system.
+
A high level of Vc2 riboswitch in the cell might cause some Vc2 Riboswitches not being able to be bind c-di-GMP molecules since these might get scarce. Therefore we used the mid-strength Anderson promoter to test this system. The RBS is already present in the Vc2 Riboswitch sequence.
  
  

Latest revision as of 11:26, 20 October 2019


Vc2 Riboswitch + sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 392
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A high level of Vc2 riboswitch in the cell might cause some Vc2 Riboswitches not being able to be bind c-di-GMP molecules since these might get scarce. Therefore we used the mid-strength Anderson promoter to test this system. The RBS is already present in the Vc2 Riboswitch sequence.


Source

These source of the part will be its components.

References