Difference between revisions of "Part:BBa K2983006"
TomZaplana (Talk | contribs) |
|||
Line 4: | Line 4: | ||
This part is a cloning adapter containing 2 SfiI restriction sites. | This part is a cloning adapter containing 2 SfiI restriction sites. | ||
+ | |||
+ | ===Usage and Biology=== | ||
+ | |||
+ | The Loop Type IIS cloning sites (triangles in Figure 1, see below for detailed information) and two SfiI sites in between to allow, if needed, the insertion of ''E. coli'' cloning selection markers like LacZalpha ([[BBa_K2448003|BBa_K2448003]]) or reporter RFP ([[BBa_J04450|BBa_J04450]]) expression cassettes. | ||
+ | |||
+ | [[File:T--Evry Paris-Saclay--General architecture of the Yarrowia lipolytica Type IIS RFC.png|900px|thumb|left|Figure 1. General architecture of the ''Yarrowia lipolytica'' Type IIS RFC[10]-compatible Loop assembly platform. ]] | ||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 11:14, 20 October 2019
SfiI cloning sites
This part is a cloning adapter containing 2 SfiI restriction sites.
Usage and Biology
The Loop Type IIS cloning sites (triangles in Figure 1, see below for detailed information) and two SfiI sites in between to allow, if needed, the insertion of E. coli cloning selection markers like LacZalpha (BBa_K2448003) or reporter RFP (BBa_J04450) expression cassettes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 12
- 1000COMPATIBLE WITH RFC[1000]