Difference between revisions of "Part:BBa K2963015"
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| − | Liu, Xiuxia , et al. "Bicistronic expression strategy for high-level expression of recombinant proteins in\r, Corynebacterium glutamicum." Engineering in Life Sciences (2017). | + | Liu, Xiuxia , et al. "Bicistronic expression strategy for high-level expression of recombinant proteins in\r, <i>Corynebacterium glutamicum</i>." Engineering in Life Sciences (2017). |
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Latest revision as of 11:13, 20 October 2019
BsracE20 - Bicistronic containing RBS20 to stabilize gene expression.
According to that pre-cistron can affect the expression of next cistron, and we select a short 5' sequence of high-protein-abundance gene from Corynebacterium glutamicum as the pre-cistron trying to improve the stabilization of the expression of racE(coding racemase enzyme).
Usage and Biology
The bicistronic has a translational coupling effect. Using bicistronics can stabilize the expression of the target gene.In order to more precisely regulate the activity of the racE gene, our team used the bicistronic system to further stabilize the expression of the racE gene.
Reference
Liu, Xiuxia , et al. "Bicistronic expression strategy for high-level expression of recombinant proteins in\r, Corynebacterium glutamicum." Engineering in Life Sciences (2017).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 998
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]