Difference between revisions of "Part:BBa K2963029"
 
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The bicistronic has a translational coupling effect. Use bicistronic can stabilize the expression of the target gene. In order to more precisely regulate the activity of the <i>racE</i> gene, we wanted to stabilize the expression of <i> racE</i> gene by using bicistronic system.
 
The bicistronic has a translational coupling effect. Use bicistronic can stabilize the expression of the target gene. In order to more precisely regulate the activity of the <i>racE</i> gene, we wanted to stabilize the expression of <i> racE</i> gene by using bicistronic system.
  
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===Reference===
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Liu, Xiuxia , et al. "Bicistronic expression strategy for high-level expression of recombinant proteins in\r, Corynebacterium glutamicum." Engineering in Life Sciences (2017).
  
 
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Latest revision as of 11:11, 20 October 2019


BsracE18000 - Bicistronic containing RBS18000 to stabilize gene expression.


Usage and Biology

The bicistronic has a translational coupling effect. Use bicistronic can stabilize the expression of the target gene. In order to more precisely regulate the activity of the racE gene, we wanted to stabilize the expression of racE gene by using bicistronic system.

Reference

Liu, Xiuxia , et al. "Bicistronic expression strategy for high-level expression of recombinant proteins in\r, Corynebacterium glutamicum." Engineering in Life Sciences (2017).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 994
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]