Difference between revisions of "Part:BBa K3165014"

 
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<partinfo>BBa_K3165014 short</partinfo>
 
<partinfo>BBa_K3165014 short</partinfo>
  
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The ccdB protein derived from the ccdAB operon in E. coli is subjected to a core mutation at the 83rd amino acid (changing Leucine to Serine) resulting in higher instability of the protein rendering it less potent for the cell.
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The ccdB protein derived from the ccdAB operon in <i>Eschereschia coli</i> is subjected to a core mutation at the 83rd amino acid (changing Leucine to Serine) resulting in higher instability of the protein rendering it less potent for the cell.
  
 
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===Usage and Biology===
 
===Usage and Biology===
  
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<h2> Biology </h2>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3165014 SequenceAndFeatures</partinfo>
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Controller of Cell Division or Death B (ccdB) is the toxic component of the <i>Eschereschia coli</i> ccdAB anti-toxin toxin system. It is a globular, dimeric protein with 101 residues per protomer, involved in the maintenance of F plasmid in cells by a mechanism involving its binding to and the poisoning of DNA Gyrase which leads to the breaking of the double-stranded DNA in the bacteria.
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The L83S mutant of the wild type ccdB protein is being mutated at the 83<sup>th</sup> amino acid residue position from Leucine to Serine in the core region of the protein.
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<h2> Usage </h2>
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<p>
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This coding sequence for ccdB L83S mutant is used with araBp <html><a  href="https://parts.igem.org/Part:BBa_K3165015">(BBa_K3165015)</a></html> upstream to regulate the production of the protein of interest only in the presence of arabinose and addition of glucose stops the leaky transcription.
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The generator of the ccdB L83S is <html><a  href="https://parts.igem.org/Part:BBa_K3165048">(BBa_K31650148)</a></html>
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</p>
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<h3> References </h3>
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<html>
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<ol>
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<li> Anusmita Sahoo, Shruti Khare, Sivasankar Devanarayanan, Pankaj C. Jain, and Raghavan Varadarajan
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Residue proximity information and protein model discrimination using saturation-suppressor mutagenesis
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doi: 10.7554/eLife.09532<br>
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</html>
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3165014 parameters</partinfo>
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<partinfo>BBa_K3165015 parameters</partinfo>
 
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Revision as of 11:09, 20 October 2019


ccdB (L83S)

The ccdB protein derived from the ccdAB operon in Eschereschia coli is subjected to a core mutation at the 83rd amino acid (changing Leucine to Serine) resulting in higher instability of the protein rendering it less potent for the cell.