Difference between revisions of "Part:BBa K2980013"

Revision as of 11:04, 20 October 2019

lacI-GFP-FUS

This part can induce asymmetric cell division in E.coli. FUSLCD in this part would aggregate at one end of cells when expressing at proper level. At the same time, lacI could attach target plasmids with lacO on it, recruiting it to the end of cell with FUSLCD. As the plasmids attracted to an end, the two filial cells would differ in the copy number of target plasmid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1377
Illegal NheI site found at 1400
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2980013 short</partinfo>
-This part can induce asymmetric cell division in E.coli. FUS-LCD in this part would aggregate at one end of cells when expressing at proper level. At the same time, lacI could attach target plasmids with lacO on it, recruiting it to the end of cell with FUS-LCD. As the plasmids attracted to an end, the two filial cells would differ in the copy number of target plasmid.
+This part can induce asymmetric cell division in E.coli. FUSLCD in this part would aggregate at one end of cells when expressing at proper level. At the same time, lacI could attach target plasmids with lacO on it, recruiting it to the end of cell with FUSLCD. As the plasmids attracted to an end, the two filial cells would differ in the copy number of target plasmid.
 <!-- Add more about the biology of this part here
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-<span class='h3bb'>Sequence and Features</span>
+===Sequence and Features===
 <partinfo>BBa_K2980013 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display
-===Functional Parameters===
+=== Functional Parameters ===
 <partinfo>BBa_K2980013 parameters</partinfo>
 <!-- -->