Difference between revisions of "Part:BBa K2980012"

Latest revision as of 11:03, 20 October 2019

Cry2-mCherry-Rluc

Rluc (Part:BBa_K1722005) was linked to the C terminal of Cry2-mCherry (Part:BBa_K2980006) and co-transformed with CIB1-GCN(4)-mEGFP-FUSLCD （Part:BBa_K2980009) to make it possible for Rluc to be recruited into phase. Then, as a control, we eliminated every component in both plasmid that may cause phase separation. Also, in order not to disturb the enzymatic activity, Rluc in both experimental group and control group were linked to mCherry. In detail, control group contained CIB1-mEGFP and mCherry-Rluc. We expected that after cultured in light, those two group would exhibit different enzymatic activity. Because phase could enrich both enzyme and substrate, bacteria in experimental group would perform higher catalytic activity compared to control group.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 393
Illegal BglII site found at 852
Illegal BamHI site found at 1331
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 277
Illegal AgeI site found at 1006
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 629
Illegal BsaI.rc site found at 38
Illegal BsaI.rc site found at 2871
Illegal SapI.rc site found at 146

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2980012 short</partinfo>
-Rluc (Part:BBa_K1722005) was linked to the C terminal of cry2-mcherry and co-transformed with CIB1-G4-GFP-FUS to make it possible for Rluc to be recruited into phase. Then, as a control, we eliminated every component in both plasmid that may cause phase separation. Also, in order not to disturb the enzymatic activity, Rluc in both experimental group and control group were linked to mcherry. In detail, control group contained CIB1-GFP and mcherry-Rluc.
+Rluc ([[Part:BBa_K1722005]]) was linked to the C terminal of Cry2-mCherry ([[Part:BBa_K2980006]]) and co-transformed with CIB1-GCN(4)-mEGFP-FUSLCD （[[Part:BBa_K2980009]]) to make it possible for Rluc to be recruited into phase. Then, as a control, we eliminated every component in both plasmid that may cause phase separation. Also, in order not to disturb the enzymatic activity, Rluc in both experimental group and control group were linked to mCherry. In detail, control group contained CIB1-mEGFP and mCherry-Rluc.
 We expected that after cultured in light, those two group would exhibit different enzymatic activity. Because phase could enrich both enzyme and substrate, bacteria in experimental group would perform higher catalytic activity compared to control group.