Difference between revisions of "Part:BBa K2996011"
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The ribosome-binding site and the translation initiation codon of this reading device are indicated. Translation of transcripts generated from the tac promoter will stop at the leader, for those two stop codons. | The ribosome-binding site and the translation initiation codon of this reading device are indicated. Translation of transcripts generated from the tac promoter will stop at the leader, for those two stop codons. | ||
The wrong reading frame and existing stop codon together will insure no expression of the florescent protein in the original situation. | The wrong reading frame and existing stop codon together will insure no expression of the florescent protein in the original situation. | ||
| − | + | <li style="display: inline-block;"> [[File:figurefilename.png|thumb|none|500px|'''Schematic representation of the integration cassettes RSRL array cloned in the pRead plasmid and strategy used for specific detection of integration events.''']] | |
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Upon information input, that is addition of corresponding inducer, spacer adaptation from cas1/2 will result in an addition of 61 base pairs, 33bp new spacer and 28bp replicated repeat, into the integration cassette. Stop codon will be moved out of frame, while EGFP in the open reading frame. | Upon information input, that is addition of corresponding inducer, spacer adaptation from cas1/2 will result in an addition of 61 base pairs, 33bp new spacer and 28bp replicated repeat, into the integration cassette. Stop codon will be moved out of frame, while EGFP in the open reading frame. | ||
Thus, successful translation of EGFP is our desired information output signal, observed by eyes and microplate reader. | Thus, successful translation of EGFP is our desired information output signal, observed by eyes and microplate reader. | ||
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tet做诱导剂的四种 | tet做诱导剂的四种 | ||
Eye PCR Microplate reader Sequencing | Eye PCR Microplate reader Sequencing | ||
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Revision as of 10:50, 20 October 2019
RSRL-eGFP downstream of pTac
The ribosome-binding site and the translation initiation codon of this reading device are indicated. Translation of transcripts generated from the tac promoter will stop at the leader, for those two stop codons.
The wrong reading frame and existing stop codon together will insure no expression of the florescent protein in the original situation.
Upon information input, that is addition of corresponding inducer, spacer adaptation from cas1/2 will result in an addition of 61 base pairs, 33bp new spacer and 28bp replicated repeat, into the integration cassette. Stop codon will be moved out of frame, while EGFP in the open reading frame. Thus, successful translation of EGFP is our desired information output signal, observed by eyes and microplate reader.
Result
tet做诱导剂的四种 Eye PCR Microplate reader Sequencing
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 252
Illegal BamHI site found at 978 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]