Difference between revisions of "Part:BBa K3183000"

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The promoter is derived from the erythromycin ribosomal methylase (ermB) promoter from the broad-host range plasmid pAM&#946;1 isolated from Enterococcus faecalis (Swinfield et al., 1990). It was characterised in six strains of <i>Lactobacillus reuteri</i> and <i>Lactococcus lactis </i>spp. <i>cremoris</i> MG1363 by <html><a href="https://academic.oup.com/femsle/article/308/1/8/513999">Lizier et al, 2010.</a>
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The promoter is derived from the erythromycin ribosomal methylase (ermB) promoter from the broad-host range plasmid pAM&#946;1 isolated from Enterococcus faecalis<sup>1</sup>. It was subsequently characterised in six strains of <i>Lactobacillus reuteri</i> and <i>Lactococcus lactis</i>spp. <i>cremoris</i> MG1363<sup>2</sup>
 
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         <!--img src="https://static.igem.org/mediawiki/2018/5/58/T--Oxford--promoters.png"alt=width="250" height="190" style="float:center">
 
         <!--img src="https://static.igem.org/mediawiki/2018/5/58/T--Oxford--promoters.png"alt=width="250" height="190" style="float:center">
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===References===
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1. Swinfield, Tracy-Jane, et al. “Physical Characterisation of the Replication Region of the Streptococcus Faecalis Plasmid pAMβ1.” Gene, vol. 87, no. 1, 1990, pp. 79–90., doi:10.1016/s0378-1119(19)30488-3.
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2. Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri  Strains.” FEMS Microbiology Letters, vol. 308, no. 1, 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.

Revision as of 10:50, 20 October 2019


Erythromycin Constitutive Promoter

P-erm is a constitutive promoter which can be used in Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species. It has also been shown to be functional in E. coli

The promoter is derived from the erythromycin ribosomal methylase (ermB) promoter from the broad-host range plasmid pAMβ1 isolated from Enterococcus faecalis1. It was subsequently characterised in six strains of Lactobacillus reuteri and Lactococcus lactisspp. cremoris MG13632


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterisation

This part was characterised in the composite part BBa_K3183101. Data for this part is shown below.