Difference between revisions of "Part:BBa K3196024"
 
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<h1>'''Characterization'''</h1>
 
<h1>'''Characterization'''</h1>
 
This is a four section for degrade and transfer lignin part.PHO1 pro is the combination of PHO1 and leading peptide from α-MF. It can help the peptide chain of VP be transferred out cells.[[File:T--HUST--China--2019-PHO1-αproVP.jpg‎|400px|thumb|center|Figure1. T--HUST--China--2019-PHO1-αproVP]]
 
This is a four section for degrade and transfer lignin part.PHO1 pro is the combination of PHO1 and leading peptide from α-MF. It can help the peptide chain of VP be transferred out cells.[[File:T--HUST--China--2019-PHO1-αproVP.jpg‎|400px|thumb|center|Figure1. T--HUST--China--2019-PHO1-αproVP]]
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<h1>'''DNA Gel Electrophoretic'''</h1>
 
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After we link PHO1 αpro and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about 1101 bp which means the PCR is successful.
 
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[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
 
  
  

Latest revision as of 10:40, 20 October 2019


AOX1-Kozak-PHO1 pro-VP-His tag-AOX1 Terminator

PHO1 αpro is a combined signal peptide, which enhance the enzyme activity 3.5 times. pelA can catalyze lignin.

Characterization

This is a four section for degrade and transfer lignin part.PHO1 pro is the combination of PHO1 and leading peptide from α-MF. It can help the peptide chain of VP be transferred out cells.
Figure1. T--HUST--China--2019-PHO1-αproVP


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 937
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]