Difference between revisions of "Part:BBa K3196022"
 
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<h1>'''Characterization'''</h1>
 
<h1>'''Characterization'''</h1>
 
This is a four section for degrade and transfer lignin part.FLO10 pro is the combination of FLO10 and leading peptide from α-MF, an efficient signal peptide with special leading peptide.<sup>[1]</sup> It can help the peptide chain of VP be transferred out cells. [[File:T--HUST--China--2019-FLO10-αproVP.jpg ‎|400px|thumb|center|Figure1. T--HUST--China--2019-FLO10-αproVP]]
 
This is a four section for degrade and transfer lignin part.FLO10 pro is the combination of FLO10 and leading peptide from α-MF, an efficient signal peptide with special leading peptide.<sup>[1]</sup> It can help the peptide chain of VP be transferred out cells. [[File:T--HUST--China--2019-FLO10-αproVP.jpg ‎|400px|thumb|center|Figure1. T--HUST--China--2019-FLO10-αproVP]]
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<h1>'''DNA Gel Electrophoretic'''</h1>
 
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After we link FLO10 αpro and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about 1101 bp which means the PCR is successful.
 
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[[File:Kozak_sequence.png ‎|400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]]
 
  
 
<h1>'''Reference'''</h1>
 
<h1>'''Reference'''</h1>

Latest revision as of 10:39, 20 October 2019


AOX1-Kozak-FLO10 pro-VP-His tag-AOX1 Terminator

FLO10 αpro is a combined signal peptide, which enhance the enzyme activity 3 times. VP can catalyze lignin.

Characterization

This is a four section for degrade and transfer lignin part.FLO10 pro is the combination of FLO10 and leading peptide from α-MF, an efficient signal peptide with special leading peptide.[1] It can help the peptide chain of VP be transferred out cells.
Figure1. T--HUST--China--2019-FLO10-αproVP

Reference

[1]Effect of signal peptides on the expression of laccase in Pichia pastoris Shi Yiping , Zhou Xue , Hu Meirong , Lin Jianhui , Tao Yong , Huang Jianzhong Acta Microbiologica Sinica 54(12) :1446 － 1452; 4 December 2014 ISSN 0001 － 6209; CN 11 － 1995 /Q

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 937
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]