Difference between revisions of "Part:BBa K3286207"
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<partinfo>BBa_K3286207 short</partinfo> | <partinfo>BBa_K3286207 short</partinfo> | ||
| − | This part is similar to part <partinfo>BBa_K3286206</partinfo>, but in this strategy, only the transmembrane domain (TMD)was used to fuse with sfGFP. So again, this part was constructed to test whether part <partinfo>BBa_K3286200</partinfo> was getting transcribed, translated and transported to the membrane. | + | This part is similar to part <partinfo>BBa_K3286206</partinfo>, but in this strategy, only the transmembrane domain (TMD) was used to fuse with sfGFP. So again, this part was constructed to test whether part <partinfo>BBa_K3286200</partinfo> was getting transcribed, translated and transported to the membrane. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 10:36, 20 October 2019
RpfCch (TMD) sfGFP fusion
This part is similar to part BBa_K3286206, but in this strategy, only the transmembrane domain (TMD) was used to fuse with sfGFP. So again, this part was constructed to test whether part BBa_K3286200 was getting transcribed, translated and transported to the membrane.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 227
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 661
- 1000COMPATIBLE WITH RFC[1000]