Difference between revisions of "Part:BBa K3245014"
 
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<h2>Characterization:</h2>
 
<h2>Characterization:</h2>
  
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<p>Please refer to </p>https://parts.igem.org/Part:BBa_K3245009
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<p>Please refer to https://parts.igem.org/Part:BBa_K3245009 </p>
  
  

Latest revision as of 10:26, 20 October 2019

Description:

This composite part is used for measurement of the new hybrid promoter. The promoter provides low leakage and a comparatively low expression level. An improved reporter protein EsfGFP ( BBa_K3245008 ) with super fluorescence can make up for the insufficiency.

Usage:

Transfer with high copy control plasmid containing luxI-luxR part ( BBa_K3245002 ) to test real time quorum sensing behavior and collect data such as OD600 and fluorescence. Transfer with plasmid containing luxR part ( BBa_C0062, etc. ) and start measuring with artificial AHL induction. It’s more suitable when accurate mechanism or dynamics research is needed.

Characterization:

Please refer to https://parts.igem.org/Part:BBa_K3245009



This composite part is used for measurement of the new promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 26
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]