Difference between revisions of "Part:BBa K3286206:Design"
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===Source=== | ===Source=== | ||
| − | See the <partinfo>K3286200</partinfo>, <partinfo>K3286203</partinfo> parts. | + | See the <partinfo>K3286200</partinfo>, <partinfo>K3286203</partinfo> parts for further information on part source. |
===References=== | ===References=== | ||
Latest revision as of 10:25, 20 October 2019
RpfCch sfGFP fusion
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 227
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 757
Illegal NgoMIV site found at 848
Illegal NgoMIV site found at 1675
Illegal NgoMIV site found at 1770
Illegal AgeI site found at 2326 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
To fuse these parts, the stopcodon of RpfCch and the startcodon of the sfGFP were removed and than fused with Golden Gate.
Source
See the BBa_K3286200, BBa_K3286203 parts for further information on part source.