Difference between revisions of "Part:BBa K3245005"
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<h2>Usage and biology:</h2> | <h2>Usage and biology:</h2> | ||
<p>This part is used to express tetR under the control of wild type quorum sensing promoter luxpR (R0062). To use it, we recommend you to couple it with BBa_K3245013 to achieve 2-step regulatory expression (luxpR-tetR-ptetR). You should clone BBa_K3245005 into a low-copy plasmid and BBa_K3245013 into a high-copy plasmid to achieve maximum regulatory effect. </p> | <p>This part is used to express tetR under the control of wild type quorum sensing promoter luxpR (R0062). To use it, we recommend you to couple it with BBa_K3245013 to achieve 2-step regulatory expression (luxpR-tetR-ptetR). You should clone BBa_K3245005 into a low-copy plasmid and BBa_K3245013 into a high-copy plasmid to achieve maximum regulatory effect. </p> | ||
Latest revision as of 10:14, 20 October 2019
Usage and biology:
This part is used to express tetR under the control of wild type quorum sensing promoter luxpR (R0062). To use it, we recommend you to couple it with BBa_K3245013 to achieve 2-step regulatory expression (luxpR-tetR-ptetR). You should clone BBa_K3245005 into a low-copy plasmid and BBa_K3245013 into a high-copy plasmid to achieve maximum regulatory effect.