Difference between revisions of "Part:BBa K3286202"

Revision as of 09:56, 20 October 2019

Vc2 Riboswitch

This is the regulatory sequence of the Vc2 Riboswitch. The Vc2 Riboswitch is a Class I c-di-GMP riboswitch. Upon binding of c-di-GMP, the downstream gene will be repressed from getting translated. When c-di-GMP is getting degraded in the cell, the alterations in structure will alleviate the repression, and translation of the gene will occur[1,2].

Vc2 Riboswitches are regulatory elements naturally occurring in Vibrio cholerea.

[1]S. Inuzuka et al., “Mutational analysis of structural elements in a class-I cyclic di-GMP riboswitch to elucidate its regulatory mechanism,” J. Biochem., vol. 160, no. 3, pp. 153–162, 2016. [2] S. Inuzuka et al., “Recognition of cyclic-di-GMP by a riboswitch conducts translational repression through masking the ribosome-binding site distant from the aptamer domain,” Genes to Cells, vol. 23, no. 6, pp. 435–447, 2018.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3286202 short</partinfo>
-This is the regulatory sequence of the Vc2 Riboswitch. The Vc2 Riboswitch is able to bind c-di-GMP as its ligand. Upon binding, the downstream gene will be repressed from getting translated. When c-di-GMP is getting degraded, the alterations in structure will alleviate the repression and translation of the gene will follow.
+This is the regulatory sequence of the Vc2 Riboswitch. The Vc2 Riboswitch is a Class I c-di-GMP riboswitch. Upon binding of c-di-GMP, the downstream gene will be repressed from getting translated. When c-di-GMP is getting degraded in the cell, the alterations in structure will alleviate the repression, and translation of the gene will occur[1,2].
+Vc2 Riboswitches are regulatory elements naturally occurring in Vibrio cholerea.
+[1]S. Inuzuka et al., “Mutational analysis of structural elements in a class-I cyclic di-GMP riboswitch to elucidate its regulatory mechanism,” J. Biochem., vol. 160, no. 3, pp. 153–162, 2016.
+[2] S. Inuzuka et al., “Recognition of cyclic-di-GMP by a riboswitch conducts translational repression through masking the ribosome-binding site distant from the aptamer domain,” Genes to Cells, vol. 23, no. 6, pp. 435–447, 2018.
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 ===Usage and Biology===
-Vc2 Riboswitches are regulatory elements naturally occurring in Vibrio cholerea.
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