Difference between revisions of "Part:BBa K3165015"
Line 20: Line 20:
 
<h2> Usage </h2>
 
<h2> Usage </h2>
 
<p>
 
<p>
−
We are using this promoter for the production of ccdB L83S mutant protein <html><a  href="https://parts.igem.org/Part:BBa_K3165014">BBa_K3165014</a></html> by induction with arabinose.
+
We are using this promoter for the production of ccdB L83S mutant protein <html><a  href="https://parts.igem.org/Part:BBa_K3165014">(BBa_K3165014)</a></html> by induction with arabinose. The Generator of ccdB L83S mutant protein <html><a  href="https://parts.igem.org/Part:BBa_K3165048">(BBa_K3165048)</a></html> which has a core muation at the 83<sup>th</sup> amino acid residue position uses this promoter as a regulator.
 
</p>
 
</p>
  
Line 26: Line 26:
 
Guzman, L. M., Belin, D., Carson, M. J. & Beckwith, J. (1995).
 
Guzman, L. M., Belin, D., Carson, M. J. & Beckwith, J. (1995).
 
Tight regulation, modulation, and high-level expression by
 
Tight regulation, modulation, and high-level expression by
−
vectors containing the arabinose PBAD promoter <br>
+
vectors containing the arabinose PBAD promoter. <br>
 
Newman, J. R. & Fuqua, C. (1999). Broad-host-range expression
 
Newman, J. R. & Fuqua, C. (1999). Broad-host-range expression
 
vectors that carry the L-arabinose-inducible Escherichia coli
 
vectors that carry the L-arabinose-inducible Escherichia coli

Revision as of 09:55, 20 October 2019


araBp

This part is used as an inducible promoter sensitive to varying concentrations of L-Arabinose. araBAD promoter is repressed by the presence of glucose and can be used to regulate gene expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 120
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Biology

The araBAD promoter of the L-Arabinose operon in Escherichia coli allows tightly controlled, titrable expression of the gene of interest placed downstream the promoter. The promoter is sensitive to small changes in the concentration of arabinose while glucose serves as a repressor. araC protein acts as the repressor protein which has an affinity to the promoter region in araBAD and suppresses the transcription of the downstream gene. The araBAD promotes has Pc subunit, O1 subunit, CAP binding subunit, I1 + I2 subunit and pBAD promoter region.

Usage

We are using this promoter for the production of ccdB L83S mutant protein (BBa_K3165014) by induction with arabinose. The Generator of ccdB L83S mutant protein (BBa_K3165048) which has a core muation at the 83th amino acid residue position uses this promoter as a regulator.

References

Guzman, L. M., Belin, D., Carson, M. J. & Beckwith, J. (1995). Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.
Newman, J. R. & Fuqua, C. (1999). Broad-host-range expression vectors that carry the L-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator.