Difference between revisions of "Part:BBa K2913013"

 
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This is a composite part consists of lldRO1-J23100-lldRO2 BBa_K1847007 and PfnrF8 BBa_K2913009. It has a functional improvement of lldRO1-J23100-lldRO2 via tansforming a specific Anderson promoter J23100 to a hypoxia-inducible promoter, PfnrF8. The lldRO1--lldRO2 was utilized with other parts of the lldPRD operon (previously named as lct), responsible for aerobic L-lactate metabolism. The lldPRD operon consists of three genes that form a single transcriptional unit inducible by growth in L-lactate. The three genes lldD, lldP and lldR encode a dehydrogenase, a permease and a regulatory protein, respectively. We chose lldR and lldP to assist building our lactic acid response unit. LldR protein can bind to the operators O1 and O2 located on each side of the PfnrF8. When lactic acid level is low, two LldR molecules will individually bind to the O1 and O2 sites and form a tetramer to make DNA strand form a hairpin structure, which can turn off the expression of the downstream gene. When lactic acid level reaches a certain point, LldR will be released from the O1 and O2 operators, and the DNA hairpin will be resolved, leading to activated transcription of the downstream gene. We also used lldP to increase the sensitivity of our system to lactic acid alteration.
 
This is a composite part consists of lldRO1-J23100-lldRO2 BBa_K1847007 and PfnrF8 BBa_K2913009. It has a functional improvement of lldRO1-J23100-lldRO2 via tansforming a specific Anderson promoter J23100 to a hypoxia-inducible promoter, PfnrF8. The lldRO1--lldRO2 was utilized with other parts of the lldPRD operon (previously named as lct), responsible for aerobic L-lactate metabolism. The lldPRD operon consists of three genes that form a single transcriptional unit inducible by growth in L-lactate. The three genes lldD, lldP and lldR encode a dehydrogenase, a permease and a regulatory protein, respectively. We chose lldR and lldP to assist building our lactic acid response unit. LldR protein can bind to the operators O1 and O2 located on each side of the PfnrF8. When lactic acid level is low, two LldR molecules will individually bind to the O1 and O2 sites and form a tetramer to make DNA strand form a hairpin structure, which can turn off the expression of the downstream gene. When lactic acid level reaches a certain point, LldR will be released from the O1 and O2 operators, and the DNA hairpin will be resolved, leading to activated transcription of the downstream gene. We also used lldP to increase the sensitivity of our system to lactic acid alteration.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2913013 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2913013 parameters</partinfo>
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Revision as of 09:27, 20 October 2019

<!doctype html> <head> <style>

  1. mw-content-text>p:nth-child(2){

} </style> </head> <body> This is a composite part consists of lldRO1-J23100-lldRO2 BBa_K1847007 and PfnrF8 BBa_K2913009. It has a functional improvement of lldRO1-J23100-lldRO2 via tansforming a specific Anderson promoter J23100 to a hypoxia-inducible promoter, PfnrF8. The lldRO1--lldRO2 was utilized with other parts of the lldPRD operon (previously named as lct), responsible for aerobic L-lactate metabolism. The lldPRD operon consists of three genes that form a single transcriptional unit inducible by growth in L-lactate. The three genes lldD, lldP and lldR encode a dehydrogenase, a permease and a regulatory protein, respectively. We chose lldR and lldP to assist building our lactic acid response unit. LldR protein can bind to the operators O1 and O2 located on each side of the PfnrF8. When lactic acid level is low, two LldR molecules will individually bind to the O1 and O2 sites and form a tetramer to make DNA strand form a hairpin structure, which can turn off the expression of the downstream gene. When lactic acid level reaches a certain point, LldR will be released from the O1 and O2 operators, and the DNA hairpin will be resolved, leading to activated transcription of the downstream gene. We also used lldP to increase the sensitivity of our system to lactic acid alteration. </body>