Difference between revisions of "Part:BBa K3132101"

Revision as of 09:19, 20 October 2019

PIP_MinimalCMV Promotor

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Sequence and Features

Assembly Compatibility:

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 <partinfo>BBa_K3132101 short</partinfo>
-This part (8*PIP MinimalCPIP_MinimalCMVMV ) is one of our synthetic promoters (SynPro) based on minimalCMV promoter (BBa_K3132003) and eight repeats of PIP binding sites. We inset the 8*PIP binding sites before the minimalCMV promoter. MinimalCMV promoter is a promoter from the vector pcDNA3.1 with very strong transcriptional activity. In this way, we can use our synthetic transcription factors (SynTFs) which is a fusing protein PIP_DBD_(G4S) linker_NLS_VP64. The PIP-DBD can bind to the 8*PIP binding sites specifically and the VP64 domain can initiate transcription by activating the MinimalCMV 8*PIP promoter. The corresponding SynTF of PIP_minimalCMV is PIP-VP64 (BBa_K3132004).
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