Difference between revisions of "Part:BBa K3088001:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 12: Line 12:
  
 
<p align="justify"><b>AAAGATGGACTGACTGAATGCCTCAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCT
 
<p align="justify"><b>AAAGATGGACTGACTGAATGCCTCAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCT
−
TAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTT
+
TAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAG
−
ATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAG
+
TGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTT
−
TGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAA
+
CGCTAAGGATGATTTCTGGAAGTGCCATTCCGCCTGACCTAGTTCAAGTGTCCGAGAAGAATTCGCGGCCGCTTCTAGAGAGGTACTGACTCATAAAAAA
−
AATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTT
+
TTTATTTGCTTTGTGAGCGGATAACAATTATAATAACGATCGCGAAAGAGGAGAAATACGCGATCGAAGATGCGTCGTCTGCGTTTTAGTCCACGCAGCT
−
CGCTAAGGATGATTTCTGGAAGTGCCATTCCGCCTGACCTAGTTCAAGTG
+
CGTTTGCACGTACGCTTTTGTTAATCGTTACATTGCTTTTTGCTTCCCTGGTCACCACTTACTTAGTTGTATTAAACTTCGCAATCTTGCCAAGTCTGCA
−
TCCGAGAAGAATTCGCGGCCGCTTCTAGAGAGGTACTGACTCATAAAAAA
+
ACAGTTTAACAAGGTATTGGCTTACGAGGTTCGCATGCTTATGACCGACAAACTTCAATTGGAAGACGGGACTCAACTGGTTGTACCACCCGCATTTCGC
−
TTTATTTGCTTTGTGAGCGGATAACAATTATAATAACGATCGCGAAAGAG
+
CGTGAGAAGTACGTTAAGGAATTGCGCGCCCCCAGTTGCATCTGTCACCCCGGATACCATGGAGAGCGCTGCCACGGCTTGTCACTGGTGCCGTTGACAG
−
GAGAAATACGCGATCGAAGATGCGTCGTCTGCGTTTTAGTCCACGCAGCT
+
AGATCCATCAGGGTGACTTTAGCCCGCTTTTCCGTTATACGTTGGCAATTATGTTACTTGCCATCGGTGGCGCATGGTTATTTATTCGCATTCAGAACCG
−
CGTTTGCACGTACGCTTTTGTTAATCGTTACATTGCTTTTTGCTTCCCTG
+
TCCGTTGGTCGATTTAGAGCACGCTGCACTTCAAGTTGGTAAGGGCATCATCCCGCCACCATTGCGCGAGTACGGGGCCTCGGAAGTACGTAGTGTTACT
−
GTCACCACTTACTTAGTTGTATTAAACTTCGCAATCTTGCCAAGTCTGCA
+
CGTGCCTTCAATCATATGGCCGCCGGTGTTAAGCAGTTAGCAGACGACCGCACTCTGTTGATGGCTGGCGGGTCTCATGATTTGCGNACCCCCTGACNCG
−
ACAGTTTAACAAGGTATTGGCTTACGAGGTTCGCATGCTTATGACCGACA
+
TATCCGCCTGGCTACTGAAATGATGTCGGAACAGGATGGAACTTGGCCGAGTCCTCAATAAAGACTTGAGGAATGAACGCTTTATTGAACGTTCTCCACT
−
AACTTCAATTGGAAGACGGGACTCAACTGGTTGTACCACCCGCATTTCGC
+
TTTGCTACCGCCCGAAANCCTTTGAANGGGACNNNATGGGGTTGGGGANAATTTTGCCAAANNGGGTCAACCGGAANNNAAAGGGCTTCCCCNNTTTTGG
−
CGTGAGAAGTACGTTAAGGAATTGCGCGCCCCCAGTTGCATCTGTCACCC
+
GGGAAAANNNNNCCCTTTTTTGNNNGGCCCTAAGGTTTAACCNNCCCCCGAAAAGGGGGTAAGGGNCCCNNNCCCCNCCNCCCGTTTTGGNNGGGAGGAC
−
CGGATACCATGGAGAGCGCTGCCACGGCTTGTCACTGGTGCCGTTGACAG
+
−
AGATCCATCAGGGTGACTTTAGCCCGCTTTTCCGTTATACGTTGGCAATT
+
−
ATGTTACTTGCCATCGGTGGCGCATGGTTATTTATTCGCATTCAGAACCG
+
−
TCCGTTGGTCGATTTAGAGCACGCTGCACTTCAAGTTGGTAAGGGCATCA
+
−
TCCCGCCACCATTGCGCGAGTACGGGGCCTCGGAAGTACGTAGTGTTACT
+
−
CGTGCCTTCAATCATATGGCCGCCGGTGTTAAGCAGTTAGCAGACGACCG
+
−
CACTCTGTTGATGGCTGGCGGGTCTCATGATTTGCGNACCCCCTGACNCG
+
−
TATCCGCCTGGCTACTGAAATGATGTCGGAACAGGATGGAACTTGGCCGA
+
−
GTCCTCAATAAAGACTTGAGGAATGAACGCTTTATTGAACGTTCTCCACT
+
−
TTTGCTACCGCCCGAAANCCTTTGAANGGGACNNNATGGGGTTGGGGANA
+
−
ATTTTGCCAAANNGGGTCAACCGGAANNNAAAGGGCTTCCCCNNTTTTGG
+
−
GGGAAAANNNNNCCCTTTTTTGNNNGGCCCTAAGGTTTAACCNNCCCCCG
+
−
AAAAGGGGGTAAGGGNCCCNNNCCCCNCCNCCCGTTTTGGNNGGGAGGAC
+
 
GNCCCCCCCCNA</b></p>
 
GNCCCCCCCCNA</b></p>
  

Revision as of 09:14, 20 October 2019


HBEGF/ENVZ Chimeric Complete


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequencing of this part (BBa_K3088001) combined with part (BBa_K769001) is confirmed and showed as follow:


AAAGATGGACTGACTGAATGCCTCAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCT TAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAG TGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTT CGCTAAGGATGATTTCTGGAAGTGCCATTCCGCCTGACCTAGTTCAAGTGTCCGAGAAGAATTCGCGGCCGCTTCTAGAGAGGTACTGACTCATAAAAAA TTTATTTGCTTTGTGAGCGGATAACAATTATAATAACGATCGCGAAAGAGGAGAAATACGCGATCGAAGATGCGTCGTCTGCGTTTTAGTCCACGCAGCT CGTTTGCACGTACGCTTTTGTTAATCGTTACATTGCTTTTTGCTTCCCTGGTCACCACTTACTTAGTTGTATTAAACTTCGCAATCTTGCCAAGTCTGCA ACAGTTTAACAAGGTATTGGCTTACGAGGTTCGCATGCTTATGACCGACAAACTTCAATTGGAAGACGGGACTCAACTGGTTGTACCACCCGCATTTCGC CGTGAGAAGTACGTTAAGGAATTGCGCGCCCCCAGTTGCATCTGTCACCCCGGATACCATGGAGAGCGCTGCCACGGCTTGTCACTGGTGCCGTTGACAG AGATCCATCAGGGTGACTTTAGCCCGCTTTTCCGTTATACGTTGGCAATTATGTTACTTGCCATCGGTGGCGCATGGTTATTTATTCGCATTCAGAACCG TCCGTTGGTCGATTTAGAGCACGCTGCACTTCAAGTTGGTAAGGGCATCATCCCGCCACCATTGCGCGAGTACGGGGCCTCGGAAGTACGTAGTGTTACT CGTGCCTTCAATCATATGGCCGCCGGTGTTAAGCAGTTAGCAGACGACCGCACTCTGTTGATGGCTGGCGGGTCTCATGATTTGCGNACCCCCTGACNCG TATCCGCCTGGCTACTGAAATGATGTCGGAACAGGATGGAACTTGGCCGAGTCCTCAATAAAGACTTGAGGAATGAACGCTTTATTGAACGTTCTCCACT TTTGCTACCGCCCGAAANCCTTTGAANGGGACNNNATGGGGTTGGGGANAATTTTGCCAAANNGGGTCAACCGGAANNNAAAGGGCTTCCCCNNTTTTGG GGGAAAANNNNNCCCTTTTTTGNNNGGCCCTAAGGTTTAACCNNCCCCCGAAAAGGGGGTAAGGGNCCCNNNCCCCNCCNCCCGTTTTGGNNGGGAGGAC GNCCCCCCCCNA


Figure 1. This the illustration of the plasmid (pSB1C3) containing part BBa K3088001 and BBa_K769001.

Source

This part was obtained by reverse translation from extensive protein modeling of HBEGF/ENVZ. The DNA sequence of both parts were obtained from National Center for Biotechnology Information (NCBI) & UniProt database

References