Difference between revisions of "Part:BBa K3088001:Design"

Revision as of 09:10, 20 October 2019

HBEGF/ENVZ Chimeric Complete

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Sequencing of this part (BBa_K3088001) combined with part (BBa_K769001) is confirmed and showed as follow:

AAAGATGGACTGACTGAATGCCTCAAATGTTCTTTACGATGCCATTGGGA TATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCT TAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTT ATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAG TGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAA AATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTT CGCTAAGGATGATTTCTGGAAGTGCCATTCCGCCTGACCTAGTTCAAGTG TCCGAGAAGAATTCGCGGCCGCTTCTAGAGAGGTACTGACTCATAAAAAA TTTATTTGCTTTGTGAGCGGATAACAATTATAATAACGATCGCGAAAGAG GAGAAATACGCGATCGAAGATGCGTCGTCTGCGTTTTAGTCCACGCAGCT CGTTTGCACGTACGCTTTTGTTAATCGTTACATTGCTTTTTGCTTCCCTG GTCACCACTTACTTAGTTGTATTAAACTTCGCAATCTTGCCAAGTCTGCA ACAGTTTAACAAGGTATTGGCTTACGAGGTTCGCATGCTTATGACCGACA AACTTCAATTGGAAGACGGGACTCAACTGGTTGTACCACCCGCATTTCGC CGTGAGAAGTACGTTAAGGAATTGCGCGCCCCCAGTTGCATCTGTCACCC CGGATACCATGGAGAGCGCTGCCACGGCTTGTCACTGGTGCCGTTGACAG AGATCCATCAGGGTGACTTTAGCCCGCTTTTCCGTTATACGTTGGCAATT ATGTTACTTGCCATCGGTGGCGCATGGTTATTTATTCGCATTCAGAACCG TCCGTTGGTCGATTTAGAGCACGCTGCACTTCAAGTTGGTAAGGGCATCA TCCCGCCACCATTGCGCGAGTACGGGGCCTCGGAAGTACGTAGTGTTACT CGTGCCTTCAATCATATGGCCGCCGGTGTTAAGCAGTTAGCAGACGACCG CACTCTGTTGATGGCTGGCGGGTCTCATGATTTGCGNACCCCCTGACNCG TATCCGCCTGGCTACTGAAATGATGTCGGAACAGGATGGAACTTGGCCGA GTCCTCAATAAAGACTTGAGGAATGAACGCTTTATTGAACGTTCTCCACT TTTGCTACCGCCCGAAANCCTTTGAANGGGACNNNATGGGGTTGGGGANA ATTTTGCCAAANNGGGTCAACCGGAANNNAAAGGGCTTCCCCNNTTTTGG GGGAAAANNNNNCCCTTTTTTGNNNGGCCCTAAGGTTTAACCNNCCCCCG AAAAGGGGGTAAGGGNCCCNNNCCCCNCCNCCCGTTTTGGNNGGGAGGAC GNCCCCCCCCNA<p>

Figure 1. This the illustration of the plasmid (pSB1C3) containing part BBa K3088001 and BBa_K769001.

Source

This part was obtained by reverse translation from extensive protein modeling of HBEGF/ENVZ. The DNA sequence of both parts were obtained from National Center for Biotechnology Information (NCBI) & UniProt database

===References===

@@ Line 11: / Line 11: @@
- <p><b>AAAGATGGACTGACTGAATGCCTCAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAA
+<p><b>AAAGATGGACTGACTGAATGCCTCAAATGTTCTTTACGATGCCATTGGGA
-AATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAAGTGCCATTCCGCCTGACCTAGTTCAAGTGTCCGAGAAGAATTCGCGGCCGCTTCTAGAGAGGTACTGA
+TATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCT
-CTCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAACGATCGCGAAAGAGGAGAAATACGCGATCGAAGATGCGTCGTCTGCGTTTTAGTCCACGCAGCTCGTTTGCACGTACGCTTTT
+TAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTT
-GTTAATCGTTACATTGCTTTTTGCTTCCCTGGTCACCACTTACTTAGTTGTATTAAACTTCGCAATCTTGCCAAGTCTGCAACAGTTTAACAAGGTATTGGCTTACGAGGTTCGCATGCTTATGACCGAC
+ATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAG
-AAACTTCAATTGGAAGACGGGACTCAACTGGTTGTACCACCCGCATTTCGCCGTGAGAAGTACGTTAAGGAATTGCGCGCCCCCAGTTGCATCTGTCACCCCGGATACCATGGAGAGCGCTGCCACG
+TGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAA
-GCTTGTCACTGGTGCCGTTGACAGAGATCCATCAGGGTGACTTTAGCCCGCTTTTCCGTTATACGTTGGCAATTATGTTACTTGCCATCGGTGGCGCATGGTTATTTATTCGCATTCAGAACCGTCCGT
+AATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTT
-TGGTCGATTTAGAGCACGCTGCACTTCAAGTTGGTAAGGGCATCATCCCGCCACCATTGCGCGAGTACGGGGCCTCGGAAGTACGTAGTGTTACTCGTGCCTTCAATCATATGGCCGCCGGTGTTAAG
+CGCTAAGGATGATTTCTGGAAGTGCCATTCCGCCTGACCTAGTTCAAGTG
-CAGTTAGCAGACGACCGCACTCTGTTGATGGCTGGCGGGTCTCATGATTTGCGNACCCCCTGACNCGTATCCGCCTGGCTACTGAAATGATGTCGGAACAGGATGGAACTTGGCCGAGTCCTCAATA
+TCCGAGAAGAATTCGCGGCCGCTTCTAGAGAGGTACTGACTCATAAAAAA
-AAGACTTGAGGAATGAACGCTTTATTGAACGTTCTCCACTTTTGCTACCGCCCGAAANCCTTTGAANGGGACNNNATGGGGTTGGGGANAATTTTGCCAAANNGGGTCAACCGGAANNNAAAGGGC
+TTTATTTGCTTTGTGAGCGGATAACAATTATAATAACGATCGCGAAAGAG
-TTCCCCNNTTTTGGGGGAAAANNNNNCCCTTTTTTGNNNGGCCCTAAGGTTTAACCNNCCCCCGAAAAGGGGGTAAGGGNCCCNNNCCCCNCCNCCCGTTTTGGNNGGGAGGACGNCCCCCCC
+GAGAAATACGCGATCGAAGATGCGTCGTCTGCGTTTTAGTCCACGCAGCT
-CNA </b>
+CGTTTGCACGTACGCTTTTGTTAATCGTTACATTGCTTTTTGCTTCCCTG
+GTCACCACTTACTTAGTTGTATTAAACTTCGCAATCTTGCCAAGTCTGCA
+ACAGTTTAACAAGGTATTGGCTTACGAGGTTCGCATGCTTATGACCGACA
+AACTTCAATTGGAAGACGGGACTCAACTGGTTGTACCACCCGCATTTCGC
+CGTGAGAAGTACGTTAAGGAATTGCGCGCCCCCAGTTGCATCTGTCACCC
+CGGATACCATGGAGAGCGCTGCCACGGCTTGTCACTGGTGCCGTTGACAG
+AGATCCATCAGGGTGACTTTAGCCCGCTTTTCCGTTATACGTTGGCAATT
+ATGTTACTTGCCATCGGTGGCGCATGGTTATTTATTCGCATTCAGAACCG
+TCCGTTGGTCGATTTAGAGCACGCTGCACTTCAAGTTGGTAAGGGCATCA
+TCCCGCCACCATTGCGCGAGTACGGGGCCTCGGAAGTACGTAGTGTTACT
+CGTGCCTTCAATCATATGGCCGCCGGTGTTAAGCAGTTAGCAGACGACCG
+CACTCTGTTGATGGCTGGCGGGTCTCATGATTTGCGNACCCCCTGACNCG
+TATCCGCCTGGCTACTGAAATGATGTCGGAACAGGATGGAACTTGGCCGA
+GTCCTCAATAAAGACTTGAGGAATGAACGCTTTATTGAACGTTCTCCACT
+TTTGCTACCGCCCGAAANCCTTTGAANGGGACNNNATGGGGTTGGGGANA
+ATTTTGCCAAANNGGGTCAACCGGAANNNAAAGGGCTTCCCCNNTTTTGG
+GGGAAAANNNNNCCCTTTTTTGNNNGGCCCTAAGGTTTAACCNNCCCCCG
+AAAAGGGGGTAAGGGNCCCNNNCCCCNCCNCCCGTTTTGGNNGGGAGGAC
+GNCCCCCCCCNA</b><p>