Difference between revisions of "Part:BBa K3088001:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 11: Line 11:
  
  
−
  <p><b>AAAGATGGACTGACTGAATGCCTCAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAA</p></b>
+
  <p><b>AAAGATGGACTGACTGAATGCCTCAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAA
−
 
+
AATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAAGTGCCATTCCGCCTGACCTAGTTCAAGTGTCCGAGAAGAATTCGCGGCCGCTTCTAGAGAGGTACTGA
−
 
+
CTCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAACGATCGCGAAAGAGGAGAAATACGCGATCGAAGATGCGTCGTCTGCGTTTTAGTCCACGCAGCTCGTTTGCACGTACGCTTTT
−
AAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAAGTGCCATTCCGCCTGACCTAGTTCAAGTGTCCGAGAAGAATTCGCGGCCGCTTCTAGAGAGGTACTGACTCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAACGATCGCGAAAGAGGAGAAATACGCGATCGAAGATGCGTCGTCTGCGTTTTAGTCCACGCAGCTCGTTTGCACGTACGCTTTTGTTAATCGTTACATTGCTTTTTGCTTCCCTGGTCACCACTTACTTAGTTGTATTAAACTTCGCAATCTTGCCAAGTCTGCAACAGTTTAACAAGGTATTGGCTTACGAGGTTCGCATGCTTATGACCGACAAACTTCAATTGGAAGACGGGACTCAACTGGTTGTACCACCCGCATTTCGCCGTGAGAAGTACGTTAAGGAATTGCGCGCCCCCAGTTGCATCTGTCACCCCGGATACCATGGAGAGCGCTGCCACGGCTTGTCACTGGTGCCGTTGACAGAGATCCATCAGGGTGACTTTAGCCCGCTTTTCCGTTATACGTTGGCAATTATGTTACTTGCCATCGGTGGCGCATGGTTATTTATTCGCATTCAGAACCGTCCGTTGGTCGATTTAGAGCACGCTGCACTTCAAGTTGGTAAGGGCATCATCCCGCCACCATTGCGCGAGTACGGGGCCTCGGAAGTACGTAGTGTTACTCGTGCCTTCAATCATATGGCCGCCGGTGTTAAGCAGTTAGCAGACGACCGCACTCTGTTGATGGCTGGCGGGTCTCATGATTTGCGNACCCCCTGACNCGTATCCGCCTGGCTACTGAAATGATGTCGGAACAGGATGGAACTTGGCCGAGTCCTCAATAAAGACTTGAGGAATGAACGCTTTATTGAACGTTCTCCACTTTTGCTACCGCCCGAAANCCTTTGAANGGGACNNNATGGGGTTGGGGANAATTTTGCCAAANNGGGTCAACCGGAANNNAAAGGGCTTCCCCNNTTTTGGGGGAAAANNNNNCCCTTTTTTGNNNGGCCCTAAGGTTTAACCNNCCCCCGAAAAGGGGGTAAGGGNCCCNNNCCCCNCCNCCCGTTTTGGNNGGGAGGACGNCCCCCCCCNA
+
GTTAATCGTTACATTGCTTTTTGCTTCCCTGGTCACCACTTACTTAGTTGTATTAAACTTCGCAATCTTGCCAAGTCTGCAACAGTTTAACAAGGTATTGGCTTACGAGGTTCGCATGCTTATGACCGAC
 +
AAACTTCAATTGGAAGACGGGACTCAACTGGTTGTACCACCCGCATTTCGCCGTGAGAAGTACGTTAAGGAATTGCGCGCCCCCAGTTGCATCTGTCACCCCGGATACCATGGAGAGCGCTGCCACG
 +
GCTTGTCACTGGTGCCGTTGACAGAGATCCATCAGGGTGACTTTAGCCCGCTTTTCCGTTATACGTTGGCAATTATGTTACTTGCCATCGGTGGCGCATGGTTATTTATTCGCATTCAGAACCGTCCGT
 +
TGGTCGATTTAGAGCACGCTGCACTTCAAGTTGGTAAGGGCATCATCCCGCCACCATTGCGCGAGTACGGGGCCTCGGAAGTACGTAGTGTTACTCGTGCCTTCAATCATATGGCCGCCGGTGTTAAG
 +
CAGTTAGCAGACGACCGCACTCTGTTGATGGCTGGCGGGTCTCATGATTTGCGNACCCCCTGACNCGTATCCGCCTGGCTACTGAAATGATGTCGGAACAGGATGGAACTTGGCCGAGTCCTCAATA
 +
AAGACTTGAGGAATGAACGCTTTATTGAACGTTCTCCACTTTTGCTACCGCCCGAAANCCTTTGAANGGGACNNNATGGGGTTGGGGANAATTTTGCCAAANNGGGTCAACCGGAANNNAAAGGGC
 +
TTCCCCNNTTTTGGGGGAAAANNNNNCCCTTTTTTGNNNGGCCCTAAGGTTTAACCNNCCCCCGAAAAGGGGGTAAGGGNCCCNNNCCCCNCCNCCCGTTTTGGNNGGGAGGACGNCCCCCCC
 +
CNA </b>
  
  

Revision as of 09:02, 20 October 2019


HBEGF/ENVZ Chimeric Complete


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequencing of this part (BBa_K3088001) combined with part (BBa_K769001) is confirmed and showed as follow:


AAAGATGGACTGACTGAATGCCTCAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAA AATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAAGTGCCATTCCGCCTGACCTAGTTCAAGTGTCCGAGAAGAATTCGCGGCCGCTTCTAGAGAGGTACTGA CTCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAACGATCGCGAAAGAGGAGAAATACGCGATCGAAGATGCGTCGTCTGCGTTTTAGTCCACGCAGCTCGTTTGCACGTACGCTTTT GTTAATCGTTACATTGCTTTTTGCTTCCCTGGTCACCACTTACTTAGTTGTATTAAACTTCGCAATCTTGCCAAGTCTGCAACAGTTTAACAAGGTATTGGCTTACGAGGTTCGCATGCTTATGACCGAC AAACTTCAATTGGAAGACGGGACTCAACTGGTTGTACCACCCGCATTTCGCCGTGAGAAGTACGTTAAGGAATTGCGCGCCCCCAGTTGCATCTGTCACCCCGGATACCATGGAGAGCGCTGCCACG GCTTGTCACTGGTGCCGTTGACAGAGATCCATCAGGGTGACTTTAGCCCGCTTTTCCGTTATACGTTGGCAATTATGTTACTTGCCATCGGTGGCGCATGGTTATTTATTCGCATTCAGAACCGTCCGT TGGTCGATTTAGAGCACGCTGCACTTCAAGTTGGTAAGGGCATCATCCCGCCACCATTGCGCGAGTACGGGGCCTCGGAAGTACGTAGTGTTACTCGTGCCTTCAATCATATGGCCGCCGGTGTTAAG CAGTTAGCAGACGACCGCACTCTGTTGATGGCTGGCGGGTCTCATGATTTGCGNACCCCCTGACNCGTATCCGCCTGGCTACTGAAATGATGTCGGAACAGGATGGAACTTGGCCGAGTCCTCAATA AAGACTTGAGGAATGAACGCTTTATTGAACGTTCTCCACTTTTGCTACCGCCCGAAANCCTTTGAANGGGACNNNATGGGGTTGGGGANAATTTTGCCAAANNGGGTCAACCGGAANNNAAAGGGC TTCCCCNNTTTTGGGGGAAAANNNNNCCCTTTTTTGNNNGGCCCTAAGGTTTAACCNNCCCCCGAAAAGGGGGTAAGGGNCCCNNNCCCCNCCNCCCGTTTTGGNNGGGAGGACGNCCCCCCC CNA

Figure 1. This the illustration of the plasmid (pSB1C3) containing part BBa K3088001 and BBa_K769001.

Source

This part was obtained by reverse translation from extensive protein modeling of HBEGF/ENVZ. The DNA sequence of both parts were obtained from National Center for Biotechnology Information (NCBI) & UniProt database

===References===