Difference between revisions of "Part:BBa K3126023"

 
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<partinfo>BBa_K3126023 short</partinfo>
 
<partinfo>BBa_K3126023 short</partinfo>
  
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Can remove nickel ions from water.
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We connected hexa-his, nixA and TgMTP1t2 with their promoters and terminators, and used KanR as resistance cassette. We characterized the ability to adsorb nickel ions of <em><span>Saccharomyces cerevisiae</span></em> which expressed this part.  We also compared its function with these three genes working in singles, pairs and the oinrigal yeast. ([[Part:BBa_K3126021]] [[Part:BBa_K3126022]][[Part:BBa_K3126020]] [[Part:BBa_K3126019]][[Part:BBa_K3126018]]).
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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Here is the curve comparing with <em><span>Saccharomyces cerevisiae</span></em> expressing [[Part:BBa_K3126021]] [[Part:BBa_K3126022]][[Part:BBa_K3126020]] [[Part:BBa_K3126019]][[Part:BBa_K3126018]]. If you want to know more about our experimental methods, please click here https://2019.igem.org/Team:HBUT-China/Notebook .
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[[File:All.png|500px|thumb|Engineering yeast comparing with these three genes working in singles, pairs and the oinrigal yeast]]
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 08:11, 20 October 2019


hexa-His-nixA-TgMTP1t2-KanR

We connected hexa-his, nixA and TgMTP1t2 with their promoters and terminators, and used KanR as resistance cassette. We characterized the ability to adsorb nickel ions of Saccharomyces cerevisiae which expressed this part. We also compared its function with these three genes working in singles, pairs and the oinrigal yeast. (Part:BBa_K3126021 Part:BBa_K3126022Part:BBa_K3126020 Part:BBa_K3126019Part:BBa_K3126018).


Usage and Biology

Here is the curve comparing with Saccharomyces cerevisiae expressing Part:BBa_K3126021 Part:BBa_K3126022Part:BBa_K3126020 Part:BBa_K3126019Part:BBa_K3126018. If you want to know more about our experimental methods, please click here https://2019.igem.org/Team:HBUT-China/Notebook .

Engineering yeast comparing with these three genes working in singles, pairs and the oinrigal yeast

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6538
    Illegal AgeI site found at 1049
    Illegal AgeI site found at 1310
  • 1000
    COMPATIBLE WITH RFC[1000]