Difference between revisions of "Part:BBa K3126021"

Revision as of 07:59, 20 October 2019

hexa-His-nixA-KanR

We connected hexa-his and nixA with their promoters and terminators, and used KanR as resistance cassette. We characterized the ability to adsorb nickel ions of Saccharomyces cerevisiae which expressed this part. We also compared its function with those two functional genes working separately (Part:BBa_K3126018 Part:BBa_K3126019).

Usage and Biology

Here is the curve comparing with Saccharomyces cerevisiae expressing Part:BBa_K3126018 and Part:BBa_K3126019. If you want to know more about our experimental methods, please click here https://2019.igem.org/Team:HBUT-China/Notebook .

Engineering yeast comparing with BBa_K3126018 and BBa_K3126019

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 4690
Illegal AgeI site found at 1049
Illegal AgeI site found at 1310
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3126021 short</partinfo>
-Can remove nickel ions from water.
+We connected hexa-his and nixA with their promoters and terminators, and used KanR as resistance cassette. We characterized the ability to adsorb nickel ions of <em><span>Saccharomyces cerevisiae</span></em> which expressed this part.  We also compared its function with those two functional genes working separately ([[Part:BBa_K3126018]] [[Part:BBa_K3126019]]).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Here is the curve comparing with <em><span>Saccharomyces cerevisiae</span></em> expressing [[Part:BBa_K3126018]] and [[Part:BBa_K3126019]]. If you want to know more about our experimental methods, please click here https://2019.igem.org/Team:HBUT-China/Notebook .
+[[File:Hh-ni-hhni.png|500px|thumb|Engineering yeast comparing with BBa_K3126018 and BBa_K3126019]]
 <!-- -->