Difference between revisions of "Part:BBa K3126018"
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<partinfo>BBa_K3126018 short</partinfo> | <partinfo>BBa_K3126018 short</partinfo> | ||
− | + | Hexa- His gene with TEF1 promoter and CYC1 terminator, using KanR as resistance cassette. We characterized the ability to adsorb nickel ions of <em><span>Saccharomyces cerevisiae</span></em> which expressed this part. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | Here is the curve comparing with original <em><span>Saccharomyces cerevisiae</span></em>. If you want to know more about our experimental methods, please click here https://2019.igem.org/Team:HBUT-China/Notebook . | ||
+ | [[File:Hexa-his_to_origin.png|500px|thumb|Engineering yeast comparing with original yeast]] | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 07:44, 20 October 2019
hexa-His-KanR
Hexa- His gene with TEF1 promoter and CYC1 terminator, using KanR as resistance cassette. We characterized the ability to adsorb nickel ions of Saccharomyces cerevisiae which expressed this part.
Usage and Biology
Here is the curve comparing with original Saccharomyces cerevisiae. If you want to know more about our experimental methods, please click here https://2019.igem.org/Team:HBUT-China/Notebook .
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2842
Illegal AgeI site found at 1049
Illegal AgeI site found at 1310 - 1000COMPATIBLE WITH RFC[1000]