Difference between revisions of "Part:BBa K3196021"
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<partinfo>BBa_K3196021 short</partinfo> | <partinfo>BBa_K3196021 short</partinfo> | ||
− | FLO10 is a Lectin-like protein with similarity to Flo1 p, thought to be expressed and involved in flocculation. VP can catalyze | + | FLO10 is a Lectin-like protein with similarity to Flo1 p, thought to be expressed and involved in flocculation. VP can catalyze lignin. |
<h1>'''Characterization'''</h1> | <h1>'''Characterization'''</h1> | ||
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<h1>'''DNA Gel Electrophoretic'''</h1> | <h1>'''DNA Gel Electrophoretic'''</h1> | ||
− | After we link FLO10 and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about | + | After we link FLO10 and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about 1101 bp which means the PCR is successful. |
[[File:Kozak_sequence.png |400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]] | [[File:Kozak_sequence.png |400px|thumb|center|Figure1. This figure shows the most possible kozak consensus sequence.]] | ||
Revision as of 07:30, 20 October 2019
AOX1-Kozak-FLO10-VP-His tag-AOX1 Terminator
FLO10 is a Lectin-like protein with similarity to Flo1 p, thought to be expressed and involved in flocculation. VP can catalyze lignin.
Characterization
This is a four section for degrade and transfer lignin part.FLO10 is a kind of signal peptide which was initially found before the protein involved in flocculation. It is supposed that the signal peptides from pichia pastoris can be better identified by its signal recognition system, thus benefiting the secretion of exogenous protein. So we add the coding sequence of FLO10 before VP to figure out whether it could increase the output of VP.
DNA Gel Electrophoretic
After we link FLO10 and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about 1101 bp which means the PCR is successful.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 937
Illegal BamHI site found at 948 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]