Difference between revisions of "Part:BBa K2997003"

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===Background===
 
===Background===
  
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The pchR gene is responsible for the production of activator proteins that can bind with the inducer (<i>p</i>-Cresol) and leads to the conformation of the protein. [1][2] In other words, the activator protein can bind with <i>p</i>-Cresol to form a protein-<i>p</i>-Cresol complex that will then bind onto the p-Cresol sensing region located just downstream of the pchR gene. The binding of the protein-<i>p</i>-Cresol complex initiates the expression of the downstream gene, in our case GFP.
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The <i>pchR</i> gene is responsible for the production of activator proteins that can bind with the inducer (<i>p</i>-Cresol) and leads to the conformation of the protein. <sup>[1][2]</sup> In other words, the activator protein can bind with <i>p</i>-Cresol to form a protein-<i>p</i>-Cresol complex that will then bind onto the <i>p</i>-Cresol sensing region located just downstream of the <i>pchR</i> gene. The binding of the protein-<i>p</i>-Cresol complex initiates the expression of the downstream gene, in our case GFP.
  
  

Latest revision as of 07:15, 20 October 2019


Sigma 54-dependent transcriptional regulator (pchR)

Background

The pchR gene is responsible for the production of activator proteins that can bind with the inducer (p-Cresol) and leads to the conformation of the protein. [1][2] In other words, the activator protein can bind with p-Cresol to form a protein-p-Cresol complex that will then bind onto the p-Cresol sensing region located just downstream of the pchR gene. The binding of the protein-p-Cresol complex initiates the expression of the downstream gene, in our case GFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1738
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1151
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1196
    Illegal AgeI site found at 179
    Illegal AgeI site found at 1079
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1105


References


  1. Cho, Ah Ra, Eun Jin Lim, Yaligara Veeranagouda, and Kyoung Lee* Department of Microbiology, Changwon National University, Changwon-si, Kyongnam 641-773, Korea . Identification of a p-Cresol Degradation Pathway by a GFP-Based Transposon in Pseudomonas and Its Dominant Expression in Colonies
  2. Merike Jo ˜esaar, Eeva Heinaru, Signe Viggor, Eve Vedler & Ain Heinaru Institute of Molecular and Cell Biology, Tartu University, Tartu, Estonia. Diversity of the transcriptional regulation of the pch gene cluster in two indigenous p-Cresol-degradative strains of Pseudomonas fluorescens