Difference between revisions of "Part:BBa K3126005"

Revision as of 07:13, 20 October 2019

hexa-His

This is a surface display system on the the yeast surface using the α-agglutinin method, it can display hexa-his on the yeast cell wall.

Usage and Biology

We use MFα1+hexa-His+ AGα1, which works together to realize the method. MFα1 can express a signal peptide that guide the fusion protein out the cell membrane. Hexa-His and AGα1 together can express hexahistidine-α-agglutinin fusion protein. It has a GPI anchor at the bottom that can stuck the C-terminal of the α-agglutinin in the cell wall, and the hexahistidine will be placed on the N-terminal. We characterized its ability to adsorb nickel ions on the surface of Saccharomyces cerevisiae cell wall.To view its function please see Part:BBa_K3126018

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 343
Illegal AgeI site found at 604
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-We use MFα1+hexa-His+ AGα1, which works together to realize the method. MFα1 can express a signal peptide that guide the fusion protein out the cell membrane. Hexa-His and AGα1 together can express hexahistidine-α-agglutinin fusion protein. It has a GPI anchor at the bottom that can stuck the C-terminal of the α-agglutinin in the cell wall, and the hexahistidine will be placed on the N-terminal. We characterized its ability to adsorb nickel ions on the surface of <em>Saccharomyces cerevisiae</em> cell wall.
+We use MFα1+hexa-His+ AGα1, which works together to realize the method. MFα1 can express a signal peptide that guide the fusion protein out the cell membrane. Hexa-His and AGα1 together can express hexahistidine-α-agglutinin fusion protein. It has a GPI anchor at the bottom that can stuck the C-terminal of the α-agglutinin in the cell wall, and the hexahistidine will be placed on the N-terminal. We characterized its ability to adsorb nickel ions on the surface of <em>Saccharomyces cerevisiae</em> cell wall.To view its function please see [[Part:BBa_K3126018]]
 [[File:Theory_of_Hexa-his.png|500px]]