Difference between revisions of "Part:BBa K3126005"
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We use MFα1+hexa-His+ AGα1, which works together to realize the method. MFα1 can express a signal peptide that guide the fusion protein out the cell membrane. Hexa-His and AGα1 together can express hexahistidine-α-agglutinin fusion protein. It has a GPI anchor at the bottom that can stuck the C-terminal of the α-agglutinin in the cell wall, and the hexahistidine will be placed on the N-terminal. We characterized its ability to adsorb nickel ions on the surface of <em>Saccharomyces cerevisiae</em> cell wall. | We use MFα1+hexa-His+ AGα1, which works together to realize the method. MFα1 can express a signal peptide that guide the fusion protein out the cell membrane. Hexa-His and AGα1 together can express hexahistidine-α-agglutinin fusion protein. It has a GPI anchor at the bottom that can stuck the C-terminal of the α-agglutinin in the cell wall, and the hexahistidine will be placed on the N-terminal. We characterized its ability to adsorb nickel ions on the surface of <em>Saccharomyces cerevisiae</em> cell wall. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 07:09, 20 October 2019
hexa-His
This is a surface display system on the the yeast surface using the α-agglutinin method, it can display hexa-his on the yeast cell wall.
Usage and Biology
We use MFα1+hexa-His+ AGα1, which works together to realize the method. MFα1 can express a signal peptide that guide the fusion protein out the cell membrane. Hexa-His and AGα1 together can express hexahistidine-α-agglutinin fusion protein. It has a GPI anchor at the bottom that can stuck the C-terminal of the α-agglutinin in the cell wall, and the hexahistidine will be placed on the N-terminal. We characterized its ability to adsorb nickel ions on the surface of Saccharomyces cerevisiae cell wall.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 343
Illegal AgeI site found at 604 - 1000COMPATIBLE WITH RFC[1000]