Difference between revisions of "Part:BBa K3279008"
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<partinfo>BBa_K3279008 short</partinfo> | <partinfo>BBa_K3279008 short</partinfo> | ||
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| + | <span class='h3bb'>Sequence and Features</span> | ||
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This part is an improvement of the previous part cenA gene (BBa_K118023). With the fusion with INP-N, the endoglucanase coded by cenA gene can be displayed on the surface of outer membrane surface of E.coli cells. | This part is an improvement of the previous part cenA gene (BBa_K118023). With the fusion with INP-N, the endoglucanase coded by cenA gene can be displayed on the surface of outer membrane surface of E.coli cells. | ||
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| + | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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[[File:CAU-China INP fused cellulases activity assays.png|500px|thumb|center|'''Fig. 4''' Enzyme activities assay (pH4.8 50℃)]] | [[File:CAU-China INP fused cellulases activity assays.png|500px|thumb|center|'''Fig. 4''' Enzyme activities assay (pH4.8 50℃)]] | ||
From the data, we summarized that the cellulases` activities were not affected remarkably with the presence of INP-N. Also, the difference of enzyme activities between the ultrasonic-disrupted samples and undisrupted samples provided another evidence of the anchoring effect of INP-N. Since the fusion protein is anchored in the outer membrane surface, which would appear in the sediments after centrifugation，the samples of suspension with fused cellulases showed the relatively low level of the activity, compared with samples of unfused ones. | From the data, we summarized that the cellulases` activities were not affected remarkably with the presence of INP-N. Also, the difference of enzyme activities between the ultrasonic-disrupted samples and undisrupted samples provided another evidence of the anchoring effect of INP-N. Since the fusion protein is anchored in the outer membrane surface, which would appear in the sediments after centrifugation，the samples of suspension with fused cellulases showed the relatively low level of the activity, compared with samples of unfused ones. | ||
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Revision as of 06:48, 20 October 2019
cenA gene fused with INP-N sequence
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 333
Illegal BamHI site found at 733 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 75
Illegal NgoMIV site found at 408
Illegal AgeI site found at 1051
Illegal AgeI site found at 1414 - 1000COMPATIBLE WITH RFC[1000]
This part is an improvement of the previous part cenA gene (BBa_K118023). With the fusion with INP-N, the endoglucanase coded by cenA gene can be displayed on the surface of outer membrane surface of E.coli cells.