Difference between revisions of "Part:BBa K3202029"
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In our study, we aimed to produce a system in which the coding sequence in the middle of the bistable system can be completely concealed. That is, there is no leakage during the normal phase of the functioning genetic circuit. Therefore, this composite part that encodes the Pc-Xyls is used as a reference of what the bacterial colony should be like is there is completely no leakage. | In our study, we aimed to produce a system in which the coding sequence in the middle of the bistable system can be completely concealed. That is, there is no leakage during the normal phase of the functioning genetic circuit. Therefore, this composite part that encodes the Pc-Xyls is used as a reference of what the bacterial colony should be like is there is completely no leakage. | ||
| − | https://2019.igem.org/ | + | https://2019.igem.org/wiki/images/1/13/T--BHSF_ND--Leakage_plasmid_2.png |
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Revision as of 06:31, 20 October 2019
Pc-Xyls
This composite part is designed as a genetic circuit that is used as a control group of our later experiment.
In our study, we aimed to produce a system in which the coding sequence in the middle of the bistable system can be completely concealed. That is, there is no leakage during the normal phase of the functioning genetic circuit. Therefore, this composite part that encodes the Pc-Xyls is used as a reference of what the bacterial colony should be like is there is completely no leakage.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 93
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 815
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 278