Difference between revisions of "Part:BBa K3202029"

Revision as of 06:30, 20 October 2019

Pc-Xyls

This composite part is designed as a genetic circuit that is used as a control group of our later experiment.

In our study, we aimed to produce a system in which the coding sequence in the middle of the bistable system can be completely concealed. That is, there is no leakage during the normal phase of the functioning genetic circuit. Therefore, this composite part that encodes the Pc-Xyls is used as a reference of what the bacterial colony should be like is there is completely no leakage.

https://2019.igem.org/File:T--BHSF_ND--Leakage_plasmid_2.png

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 93
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 815
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 278

@@ Line 7: / Line 7: @@
 In our study, we aimed to produce a system in which the coding sequence in the middle of the bistable system can be completely concealed. That is, there is no leakage during the normal phase of the functioning genetic circuit. Therefore, this composite part that encodes the Pc-Xyls is used as a reference of what the bacterial colony should be like is there is completely no leakage.
-https://2019.igem.org/wiki/images/3/3f/T--BHSF_ND--sheyingxin.png
+https://2019.igem.org/File:T--BHSF_ND--Leakage_plasmid_2.png
-https://2019.igem.org/parts/images/3/3f/T--BHSF_ND--toxinplasmid1.png
 <!-- Add more about the biology of this part here