Difference between revisions of "Part:BBa K2910006"
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<partinfo>BBa_K2910006 short</partinfo> | <partinfo>BBa_K2910006 short</partinfo> | ||
| − | + | This part encodes ''Ideonella sakaiensis'' poly(ethylene terephthalate) hydrolase with several mutations predicted to increase its catalytic activity. The bulky arginine residue at 280 has been mutated to threonine, to reduce steric clash with PET and to contribute a hydrogen-bonding interaction with ester oxygen in the PET chain. Mutations at sites 208, 238, and 245 are predicted to increase interaction between isPETase and aromatic moieties of PET. T88 was mutated to isoleucine to better interact with a nonpolar PET moiety. N241 was mutated to threonine, to better-position a hydrogen-bonding interaction with ester oxygen in the PET chain. | |
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| + | Internal working name: Dimi4 | ||
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Latest revision as of 06:11, 20 October 2019
IsPETase-T88I-I208Y-S238F-N241T-S245Y-R280T with C-terminal Hexahistidine Tag
This part encodes Ideonella sakaiensis poly(ethylene terephthalate) hydrolase with several mutations predicted to increase its catalytic activity. The bulky arginine residue at 280 has been mutated to threonine, to reduce steric clash with PET and to contribute a hydrogen-bonding interaction with ester oxygen in the PET chain. Mutations at sites 208, 238, and 245 are predicted to increase interaction between isPETase and aromatic moieties of PET. T88 was mutated to isoleucine to better interact with a nonpolar PET moiety. N241 was mutated to threonine, to better-position a hydrogen-bonding interaction with ester oxygen in the PET chain.
Internal working name: Dimi4
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 67
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]