Difference between revisions of "Part:BBa K1255002:Design"

 
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===References===
 
===References===
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<p>CIDEB-UANL_Mexico. (2014). Capture module. Accessed 24 April 2019, < http://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_capture>.</p>
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<p>Huang, L. (2010). Growth kinetics of Escherichia coli O157:H7 in mechanically-tenderized beef. International Journal of Food Microbiology. No. 140, pp. 40–48.</p>
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<p>Krulwich, T.& Ivey, M. (1994) Sodium ion binding proteins. Accessed 24 April 2019, < http://www.google.com.mx/patents/US5346815>.</p>

Latest revision as of 05:03, 20 October 2019


NhaS with an RFP reporter induced by an UV promoter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 890
    Illegal AgeI site found at 1002
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NhaS (BBa_K1255000) sequence was optimized for its expression in E. coli


Source

RFP comes from Discosoma striata (coral) and NhaS from Bacillus firms

References

CIDEB-UANL_Mexico. (2014). Capture module. Accessed 24 April 2019, < http://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_capture>.

Huang, L. (2010). Growth kinetics of Escherichia coli O157:H7 in mechanically-tenderized beef. International Journal of Food Microbiology. No. 140, pp. 40–48.

Krulwich, T.& Ivey, M. (1994) Sodium ion binding proteins. Accessed 24 April 2019, < http://www.google.com.mx/patents/US5346815>.