Difference between revisions of "Part:BBa K2910004"
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<partinfo>BBa_K2910004 short</partinfo> | <partinfo>BBa_K2910004 short</partinfo> | ||
| − | + | This part encodes ''Ideonella sakaiensis'' poly(ethylene terephthalate) hydrolase with several mutations predicted to increase its catalytic activity. The bulky arginine residue at 280 has been mutated to threonine, to reduce steric clash with PET and to contribute a hydrogen-bonding interaction with ester oxygen in the PET chain. Mutations at sites 208, 238, and 245 are predicted to increase interaction between isPETase and aromatic moieties of PET. T88 was mutated to isoleucine to better interact with a nonpolar PET moiety. | |
| + | |||
| + | Internal working name: Dimi2 | ||
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Latest revision as of 04:58, 20 October 2019
IsPETase-T88I-I208F-S238F-S245F-R280T with C-terminal Hexahistidine Tag
This part encodes Ideonella sakaiensis poly(ethylene terephthalate) hydrolase with several mutations predicted to increase its catalytic activity. The bulky arginine residue at 280 has been mutated to threonine, to reduce steric clash with PET and to contribute a hydrogen-bonding interaction with ester oxygen in the PET chain. Mutations at sites 208, 238, and 245 are predicted to increase interaction between isPETase and aromatic moieties of PET. T88 was mutated to isoleucine to better interact with a nonpolar PET moiety.
Internal working name: Dimi2
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 67
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]