Difference between revisions of "Part:BBa K3093003:Design"

Revision as of 04:50, 20 October 2019

chbB promoter with a new -35

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The chbB promotor was obtained from E.coli MG1655 through PCR. And we remove the strong NagC binding site and change the old -35 signal "TACTAT" to the new -35 signal "TTGACA"

Source

Escherichia coli str. K-12 substr.MG1655.

References

[1]Plumbridge J, Pellegrini O. Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP. Mol Microbiol. 2004 Apr;52(2):437-49. PubMed PMID: 15066032.

Revision as of 04:30, 20 October 2019 (view source) Registry (Talk \| contribs)		Revision as of 04:50, 20 October 2019 (view source) Still Water (Talk \| contribs) (→‎References) Newer edit →
Line 16:		Line 16:

	===References===		===References===
		+	[1]Plumbridge J, Pellegrini O. Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP. Mol Microbiol. 2004 Apr;52(2):437-49. PubMed PMID: 15066032.