Difference between revisions of "Part:BBa K3126005"

 
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<partinfo>BBa_K3126005 short</partinfo>
 
<partinfo>BBa_K3126005 short</partinfo>
  
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The gene can express the protein that can combine Nickel ions.
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This is a surface display system on the the yeast surface using the α-agglutinin method, it can display hexa-his on the yeast cell wall.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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We use MFα1+hexa-His+ AGα1, which works together to realize the method. MFα1 can express a signal peptide that guide the fusion protein out the cell membrane. Hexa-His and AGα1 together can express hexahistidine-α-agglutinin fusion protein. It has a GPI anchor at the bottom that can stuck the C-terminal of the α-agglutinin in the cell wall, and the hexahistidine will be placed on the N-terminal. We characterized its ability to adsorb nickel ions on the surface of <em>Saccharomyces cerevisiae</em> cell wall.
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src="https://static.igem.org/mediawiki/parts/7/73/Theory_of_Hexa-his.png" width="615" height="228"/> </span>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 04:28, 20 October 2019


hexa-His

This is a surface display system on the the yeast surface using the α-agglutinin method, it can display hexa-his on the yeast cell wall.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 343
    Illegal AgeI site found at 604
  • 1000
    COMPATIBLE WITH RFC[1000]