Difference between revisions of "Part:BBa K3278000"

Revision as of 03:59, 20 October 2019

pDawn-mEGFP

This part can characterize the light control efficiency of pDawn system. PDawn is a blue light control system which can start the expression of the target gene. With the target gene usually being proteins or RNAs, it may be relatively difficult to characterize its expression level. Thus, we construct the pDawn+mEGFP by using homologous recombination to show the light control efficiency by fluorescence intensity. Registers can use this part in their own plasmid and bacteria to test its actual efficiency in varied situation.

Fig1. pDawn-mEGFP-0 hour

Fig2. pDawn-mEGFP-6 hour

Fig2. pDawn-mEGFP-12 hour

Fig2. pDawn-mEGFP-24 hour

Fig2. pDawn-mEGFP-30 hour

Fig2. pDawn-mEGFP-36 hour

To see more changes on pDawn, go to the websites below.

pDawn+mEGFP: BBa_K3278000

pDawn with T7 promoter: BBa_3278001

pDawn with mtac promoter: BBa_K3278002 (good)

pdawn with tac promoter: BBa_K3278005

pDawn with YF2: BBa_K3278006 (good)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2148
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 46
Illegal NgoMIV site found at 178
Illegal NgoMIV site found at 272
Illegal NgoMIV site found at 565
Illegal NgoMIV site found at 1059
Illegal NgoMIV site found at 1077
Illegal NgoMIV site found at 1167
Illegal AgeI site found at 397
Illegal AgeI site found at 1525
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1620
Illegal BsaI.rc site found at 508

@@ Line 14: / Line 14: @@
 <center>Fig2. pDawn-mEGFP-12 hour </center>
 <br>
-[[Image:pme24h.png|400px|center|pDawn-mEGFP-24 hour]]
+[[Image:pme_24h.png|400px|center|pDawn-mEGFP-24 hour]]
 <center>Fig2. pDawn-mEGFP-24 hour </center>
 <br>