Difference between revisions of "Part:BBa K150009"

Revision as of 21:05, 21 October 2008

ColicinE1 Producer Controlled by 3OC6HSL Receiver Device

The constitutive expressed LuxR protein is able to build a complex with the AHL molecule. This complex function as transcription factor for the lux pR promoter and activates by this the colicinE1 production. This activation of the lux pR promoter can additionally lead to expression of the kil protein by read through of the terminator t1 and therefore to lysis of the cell and colicin release. The colicinE1 immunity protein (imm) located on the reverse strand is expressed under a constitutive promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1083
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1004
Illegal SapI site found at 1389

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 __NOTOC__
 <partinfo>BBa_K150009 short</partinfo>
-The constitutive expressed LuxR protein is able to build a complex with the AHL molecule. This complex function as transcription factor for the lux pR promoter and activates by this the colicinE1 production. This activation of the lux pR promoter can additionally lead to expression of the kil protein by read through of the terminator t1 and therefore to lysis of the cell and colcin release. The colicinE1 immunity protein (imm) lying on the reverse strand is expressend under a constitutive promotor.
+The constitutive expressed LuxR protein is able to build a complex with the AHL molecule. This complex function as transcription factor for the lux pR promoter and activates by this the colicinE1 production. This activation of the lux pR promoter can additionally lead to expression of the kil protein by read through of the terminator t1 and therefore to lysis of the cell and colicin release. The colicinE1 immunity protein (imm) located on the reverse strand is expressed under a constitutive promoter.
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