Difference between revisions of "Part:BBa K3179103"

Latest revision as of 03:47, 20 October 2019

pUbC-rtTA3

Usage and Biology

This part consists of promoter pUbC and basic part rtA3. pUbC from human ubiquitin C gene and is a mammalian promoter. pUbC is usually used for regular expression and has a lower expression. rtTA is a key component of Tet-On system, and rtTA3 is third generation rtTA. rtTA3 is an activation factor of pTRE. rtTA3 isn’t active when it is present alone, and it can be active when combine with doxycycline (DOX), then the complex can combine to pTRE and start the downstream transcription. When DOX get removed the rtTA3 will became no activation again.
In our experiment, pUbC-rtTA3 is used to test the effect of the influence of different promoter to the expression of rtTA3.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 999
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 203
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 999
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 999
Illegal NgoMIV site found at 473
Illegal AgeI site found at 209
1000
COMPATIBLE WITH RFC[1000]

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 pUbC-rtTA3
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This part consists of promoter pUbC and basic part rtA3. pUbC from human ubiquitin C gene and is a mammalian promoter. pUbC is usually used for regular expression and has a lower expression. rtTA is a key component of Tet-On system, and rtTA3 is third generation rtTA. rtTA3 is an activation factor of pTRE. rtTA3 isn’t active when it is present alone, and it can be active when combine with doxycycline (DOX), then the complex can combine to pTRE and start the downstream transcription. When DOX get removed the rtTA3 will became no activation again.<br style="clear: both" />
+In our experiment, pUbC-rtTA3 is used to test the effect of the influence of different promoter to the expression of rtTA3.
 <!-- -->