Difference between revisions of "Part:BBa K150010"

Latest revision as of 21:00, 21 October 2008

ColicinE9 operon

This colicinE9 operon contains all genes from the pColE9-J plasmid. The colE9 gene codes for the lethal colicinE9 protein. The gene for the colE9 immunity protein is responsible for the immunity of the producing host bacteria to the colicinE9 and is therefore controlled by a constitutive promoter. Expression of the atypical lysis protein leads finally to the lysis of the host bacteria and thereby to the release of the colicinE9. Additionally the pColE9-J plasmid contains a colE5 immunity protein and a canonical lysis protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K150010 short</partinfo>
-This colicinE1 operon contains all three important genes for colicinE9 expression and release. The colE9 gene codes for the lethal colicinE9 protein. The gene for the colE9 immunity protein is responsible for the immunity of the producing host bacteria to the colicinE9 and is therefore controlled by a constitutive promoter. Expression of the atypical lysis protein leads finally to the lysis of the host bacteria and thereby to the release of the colicinE9.
+This colicinE9 operon contains all genes from the pColE9-J plasmid. The colE9 gene codes for the lethal colicinE9 protein. The gene for the colE9 immunity protein is responsible for the immunity of the producing host bacteria to the colicinE9 and is therefore controlled by a constitutive promoter. Expression of the atypical lysis protein leads finally to the lysis of the host bacteria and thereby to the release of the colicinE9. Additionally the pColE9-J plasmid contains a colE5 immunity protein and a canonical lysis protein.
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