Difference between revisions of "Part:BBa K2922026"
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===Summary===
 
===Summary===
 
Abbreviated as Ekil, lysis protein for Colicin-E1 is a 4.8 kDa protein, which is encoded by gene <i>kil</i>.The lysis protein is required for both Colicin-E1 release and partial cell lysis. [1] (Fig.1)
 
Abbreviated as Ekil, lysis protein for Colicin-E1 is a 4.8 kDa protein, which is encoded by gene <i>kil</i>.The lysis protein is required for both Colicin-E1 release and partial cell lysis. [1] (Fig.1)
−
<table><tr><th>[[File:E1design.png|thumb|300px|Fig.1 Ekil helps Colicin-E1 release and makes partial cell lysis.]]</th><th></table>
+
<table><tr><th>[[File:E1design.png|thumb|720px|Fig.1 Ekil helps Colicin-E1 release and makes partial cell lysis.]]</th><th></table>
 
===Identification===
 
===Identification===
−
When we are building this circuit, we are doing the nucleic acid gel electrophoresis experiment to verify. After the circuit is built, we send the plasmid to sequence, and get the correct sequencing.
+
When we were building this circuit, we did the nucleic acid gel electrophoresis experiment to verify. After the circuit was built, we sent the plasmid to sequence, and got the correct sequencing.
−
After new molecular cloning experiments, we do Enzyme-Cut identification to certify the plasmid is correct. We use the <i>Xba</i>I and <i>Pst</i>I to cut the plasmid, then we getting the target separate fragment-138bp. (Fig.2)
+
After new molecular cloning experiments, we did Enzyme-Cut identification to certify the plasmid was correct. We used the <i>Xba</i>I and <i>Pst</i>I to cut the plasmid, then we got the target separate fragment-138bp. (Fig.2)
 
<table><tr><th>[[File:Nkil-Ekil.png|thumb|300px|Fig.2 The result of this plasmid cut with enzyme <i>Xba</i>I and <i>Pst</i>I.]]</th><th></table>
 
<table><tr><th>[[File:Nkil-Ekil.png|thumb|300px|Fig.2 The result of this plasmid cut with enzyme <i>Xba</i>I and <i>Pst</i>I.]]</th><th></table>
 
===Reference===
 
===Reference===

Revision as of 02:59, 20 October 2019


Lysis protein for colicin-E1 coding region

Summary

Abbreviated as Ekil, lysis protein for Colicin-E1 is a 4.8 kDa protein, which is encoded by gene kil.The lysis protein is required for both Colicin-E1 release and partial cell lysis. [1] (Fig.1)

Fig.1 Ekil helps Colicin-E1 release and makes partial cell lysis.

Identification

When we were building this circuit, we did the nucleic acid gel electrophoresis experiment to verify. After the circuit was built, we sent the plasmid to sequence, and got the correct sequencing. After new molecular cloning experiments, we did Enzyme-Cut identification to certify the plasmid was correct. We used the XbaI and PstI to cut the plasmid, then we got the target separate fragment-138bp. (Fig.2)

Fig.2 The result of this plasmid cut with enzyme XbaI and PstI.

Reference

[1] E. Cascales et al., Colicin biology. Microbiol Mol Biol Rev 71, 158-229 (2007).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 118
  • 1000
    COMPATIBLE WITH RFC[1000]