Difference between revisions of "Part:BBa K1179016"
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=2019 SYSU-CHINA's characterization= | =2019 SYSU-CHINA's characterization= | ||
| − | + | SYSU-CHINA added some quantitative experimental characterization data on the L7Ae protein. | |
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L7Ae is a protein targets RNA K-turn. We characterizated L7Ae and constructed an L7Ae mutation（L7AeO,BBa_3179001) to study the suppression efficiency of the L7Ae-Kturn System. | L7Ae is a protein targets RNA K-turn. We characterizated L7Ae and constructed an L7Ae mutation（L7AeO,BBa_3179001) to study the suppression efficiency of the L7Ae-Kturn System. | ||
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[[File:L7AeO.jpg]] | [[File:L7AeO.jpg]] | ||
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| + | [[File:T-SYSU-CHINA-L7Ae-Kturn.jpg|500px|thumb|left]] | ||
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| − | + | =Cytotoxicity test= | |
| − | + | SYSU-CHINA tested the cytotoxicity of L7Ae, which was previously considered to be slightly cytotoxic, but the opposite result was found in our experiments.Neither L7Ae nor its mutants showed significant cytotoxicity. | |
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[[File:T-SYSU-CHINA-T-SYSU-CHINA-cell_experiment.jpg]] | [[File:T-SYSU-CHINA-T-SYSU-CHINA-cell_experiment.jpg]] | ||
Revision as of 02:42, 20 October 2019
L7Ae (Transcriptional level repressor)
L7Ae is a transcriptional level repressor which targets RNA K-turns.
2019 SYSU-CHINA's characterization
SYSU-CHINA added some quantitative experimental characterization data on the L7Ae protein.
L7Ae is a protein targets RNA K-turn. We characterizated L7Ae and constructed an L7Ae mutation（L7AeO,BBa_3179001) to study the suppression efficiency of the L7Ae-Kturn System.
The result shows that the mutation couldn’t work normally but still functions in high concentration. Meanwhile, the 1000ng/ml L7Ae can depress the translation of mRNA with a efficiency of 84%.
Now the sequence analysis and computer modeling have shown the predicted structure of L7Ae. We can easily see that there are some α-helixes in L7Ae, and one of them is the bidding site.
Cytotoxicity test
SYSU-CHINA tested the cytotoxicity of L7Ae, which was previously considered to be slightly cytotoxic, but the opposite result was found in our experiments.Neither L7Ae nor its mutants showed significant cytotoxicity.
File:T-SYSU-CHINA-T-SYSU-CHINA-cell experiment.jpg
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]