Difference between revisions of "Part:BBa K1179016"
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Revision as of 02:32, 20 October 2019
L7Ae (Transcriptional level repressor)
L7Ae is a transcriptional level repressor which targets RNA K-turns.
2019 SYSU-CHINA's characterization
We added some quantitative experimental characterization data on the L7Ae protein.
L7Ae is a protein targets RNA K-turn. We characterizated L7Ae and constructed an L7Ae mutation（L7AeO,BBa_3179001) to study the suppression efficiency of the L7Ae-Kturn System.
The result shows that the mutation couldn’t work normally but still functions in high concentration. Meanwhile, the 1000ng/ml L7Ae can depress the translation of mRNA with a efficiency of 84%.
File:T-SYSU-CHINA-structure-of-L7Ae-Kturn.jpg
Now the sequence analysis and computer modeling have shown the predicted structure of L7Ae. We can easily see that there are some α-helixes in L7Ae, and one of them is the bidding site.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]