Difference between revisions of "Part:BBa K1179016"

 
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=2019 SYSU-CHINA's characterization=
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We added some quantitative experimental characterization data on the L7Ae protein.
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L7Ae is a protein targets RNA K-turn. We characterizated L7Ae and constructed an L7Ae mutation（L7AeO,BBa_3179001) to study the suppression efficiency of the L7Ae-Kturn System.
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[[File:T-SYSU-CHINA-L7AeO mutate site.jpg]]
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The result shows that the mutation couldn’t work normally but still functions in high concentration. Meanwhile, the 1000ng/ml L7Ae can depress the translation of mRNA with a efficiency of 84%.
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[[File:L7AeO.jpg]]
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[[File:T-SYSU-CHINA-structure-of-L7Ae-Kturn.jpg|500px|thumb|left]]
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Now the sequence analysis and computer modeling have shown the predicted structure of L7Ae. We can easily see that there are some α-helixes in L7Ae, and one of them is the bidding site.
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[[File:T-SYSU-CHINA-structure-of-L7Ae.png|500px|thumb|left]]
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===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 02:28, 20 October 2019


L7Ae (Transcriptional level repressor)

L7Ae is a transcriptional level repressor which targets RNA K-turns.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]