Difference between revisions of "Part:BBa K3165042"
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Revision as of 01:52, 20 October 2019


CcaS-Gp2 + Ho1-PcyA-CcaR

This part consists of all the components of the CcaS-CcaR optogenetic system with the bacteriostatic growth-inhibitory protein Gp2 downstream of the pCpcG2 promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2247
    Illegal NheI site found at 2270
    Illegal NheI site found at 3998
    Illegal NheI site found at 4021
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

CcaSR is a green-light activated two-component signal transduction system (TCS) that controls the expression of phycobilisome linker protein CpcG2 in Synechocystis sp. PCC6803 complementary chromatic adaptation pathway. The naturally occurring CcaS protein is a cyanobacteriochrome sensor histidine kinase consisting of an N-terminal GAF domain (cGMP phosphodiesterase/ adenyl cyclase/ FhlA) bound to two PAS domains of unknown function attached to a C-terminal Histidine Kinase domain. Upon activation with green light, the CcaS is autophosphorylated to a red-absorbing conformation that undergoes phosphotransfer to the response regulator CcaR leading to the activation of the PcpcG2 promoter initiating transcription of the gene of interest.
In this gene construct, the T7 phage protein Gp2 is used as the gene of interest which has a bacteriostatic effect by its affinity for the sigma^70 subunit of the host RNA Polymerase causing misappropriation of the holoenzyme inhibiting transcription.
This part is used as a light-mediated growth regulatory system to dynamically control bacterial cell populations.