Difference between revisions of "Part:BBa K3165041"

 
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This part consists of the mini-CcaS protein complex with ECFP under the pCpcG2 promoter.
 
This part consists of the mini-CcaS protein complex with ECFP under the pCpcG2 promoter.
  
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><b>Sequence and Features</b></span>
 
<partinfo>BBa_K3165041 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3165041 SequenceAndFeatures</partinfo>
  
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===Usage and Biology===
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The mini-CcaS protein consisting of a GAF domain, two PAS domains and a histidine kinase domain forms the light-sensitive part of the CcaS-CcaR system. ECFP placed downstream of the pCpcG2 is transcribed upon activation of the promoter by the response regulator CcaR mediated by the histidine kinase domain of CcaS. This part when used in accompaniment with the CcaR and Ho1-PcyA system will result in the translation of the complete photo-sensitive system.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 01:35, 20 October 2019


mini-CcaS + ECFP

This part consists of the mini-CcaS protein complex with ECFP under the pCpcG2 promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

The mini-CcaS protein consisting of a GAF domain, two PAS domains and a histidine kinase domain forms the light-sensitive part of the CcaS-CcaR system. ECFP placed downstream of the pCpcG2 is transcribed upon activation of the promoter by the response regulator CcaR mediated by the histidine kinase domain of CcaS. This part when used in accompaniment with the CcaR and Ho1-PcyA system will result in the translation of the complete photo-sensitive system.