Difference between revisions of "Part:BBa K3165040"

 
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This part places the fluorescent protein - ECFP under the inducible pCpcG2 promoter.
 
This part places the fluorescent protein - ECFP under the inducible pCpcG2 promoter.
  
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><b>Sequence and Features</b></span>
 
<partinfo>BBa_K3165040 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3165040 SequenceAndFeatures</partinfo>
  
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===Usage and Biology===
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This part encodes for the engineered cyan fluorescent protein (ECFP) under an inducible promoter pCpcG2. The promoter is activated by the phosphorylated form of CcaR (a response regulator) and deactivated by dephosphorylated CcaR. the phosphorylation/dephosphorylation of CcaR depends on the light that is shone and perceived by the trans-membrane CcaS protein.
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Upon light stimulation, the production of ECFP is triggered leading to a fluorescence by the bacteria. By fluorescence measurements, the functioning of the light-sensitive system can be quantified
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This part is used in association with the CcaS-CcaR system as a light-induced system to quantify the protein expression.
  
 
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Revision as of 01:28, 20 October 2019


ECFP under PcpcG2 expression

This part places the fluorescent protein - ECFP under the inducible pCpcG2 promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

This part encodes for the engineered cyan fluorescent protein (ECFP) under an inducible promoter pCpcG2. The promoter is activated by the phosphorylated form of CcaR (a response regulator) and deactivated by dephosphorylated CcaR. the phosphorylation/dephosphorylation of CcaR depends on the light that is shone and perceived by the trans-membrane CcaS protein. Upon light stimulation, the production of ECFP is triggered leading to a fluorescence by the bacteria. By fluorescence measurements, the functioning of the light-sensitive system can be quantified This part is used in association with the CcaS-CcaR system as a light-induced system to quantify the protein expression.