Difference between revisions of "Part:BBa K103001"
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AID protein | AID protein | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | *AID (Activation Induced Deamidase) is a protein active in mammal lymphocytes, where it causes the somatic hypermutation – an increase of mutation level in antibody coding sequences. There is a publication [http://www.ncbi.nlm.nih.gov/pubmed/12097915?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum] which proves AID activity in E. coli cells. AID acts on single-stranded DNA, so only highly transcribed loci are mutated. | |
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+ | *We have used AID to create 'one-armed bandit' strain that mutates only selected gene / set of genes. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K103001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K103001 SequenceAndFeatures</partinfo> |
Revision as of 19:04, 21 October 2008
AID protein
AID protein
Usage and Biology
- AID (Activation Induced Deamidase) is a protein active in mammal lymphocytes, where it causes the somatic hypermutation – an increase of mutation level in antibody coding sequences. There is a publication [http://www.ncbi.nlm.nih.gov/pubmed/12097915?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum] which proves AID activity in E. coli cells. AID acts on single-stranded DNA, so only highly transcribed loci are mutated.
- We have used AID to create 'one-armed bandit' strain that mutates only selected gene / set of genes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 78
Illegal SapI site found at 179